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"Reverse transcription-PCR kit" for first strand cDNA synthesis
The SCRIPTUM FIRST cDNA synthesis kit includes all reagents needed for the first strand cDNA synthesis, and combines ease of use with high flexibility.
A reverse transcriptase in premium quality, ultra-pure dNTPs and an optimized reaction buffer ensure superior results with highest reproducibility. The kit is optimized for high efficiency for a wide range of primer-template combinations. The SCRIPTUM FIRST reverse transcriptase is a recombinantly produced version of the M-MLV reverse transcriptase (M-MLV RT), wherein the eliminated RNase H activity and the thermal stability was improved.
Application
Synthesis of highly structured and long cDNA fragments, extremely sensitive and highly specific RT PCR, DNA labeling. The kit contains both so-called random hexamer primers and oligo (dT) 20 primer. Optionally, gene-specific primers can be used. Here, however, we recommand using the "Scriptum high precision " as an alternative cDNA synthesis kit ".
Product | Article No. | Quantity |
---|---|---|
SCRIPTUM First strand | BS.48.020 | 20 reactions in 20 μl |
BS.48.100 | 100 reactions in 20 μl | |
BS.48.500 | 500 reactions in 20 μl |
Protocols
High-precision one-step reverse transcription-PCR kit for the preparation of cDNA
SCRIPTUM High Precise is a mix for an extremely precise and also a very quick preparation of cDNA.
The kit produces a highly sensitive and specific reverse transcription followed by PCR comfortably in just one tube.
The SCRIPTUM high precise kit reaches its high specificity by a genetically engineered reverse transcriptase with improved thermal stability. Additionally the kit includes a genetically produced Taq DNA polymerase with proof reading activity. The enzyme works 2x as fast as a normal Taq polymerase.
The kit reaches a 50-fold higher accuracy and is twice as fast as a conventional elongation cDNA Kit.
The SCRIPTUM High Precise Kit contains all the necessary reagents for RT-PCR (reverse transcription followed by PCR) except template and primer.
The handling in just one reaction tube and the few pipetting steps make the kit extremely comfortable and provides very fast excellent results.
Product | Article No. | Quantity |
---|---|---|
SCRIPTUM High precise | BS.50.020 | 50 reactions in 20 μl |
BS.50.100 | 250 reactions in 20 μl | |
BS.50.500 | 1250 reactions in 20 μl |
Protocols
Bio&SELL SCRIPTUM enzyme mixes for the preparation of cDNA
The Bio&SELL SCRIPTUM one-step kits for the preparation of cDNA can offer an excellent way to produce fast, convenient and very cheap cDNA.
The SCRIPTUM mixes consist of a reverse transcriptase and a Taq DNA polymerase, respectively. Both enzymes are genetically optimized so that:
- high specificities are achieved
- high cDNA yields are guaranteed
- and the transcription efficiency is optimally ensured even with strong secondary structures.
Target sequences can generally be detected in 1 pg to 20 ng poly (A) RNA (mRNA) or 10/50 pg to 1 μg of total RNA. High expressed target sequences may already be successfully amplified from lower amounts of RNA.
The SCRIPTUM High Precise kit includes a Taq DNA polymerase with proofreading activity. The SCRIPTUM Standard Mix contains a "normal" Taq DNA Ploymerase.
After putting together all reaction ingredients the two reactions "reverse transcription" and "PCR" are carried out in just one tube. (Procedure see data sheet). This saves you time and reduces the risc of contamination, especially when the same target sequence is analyzed in many different RNA samples.
"One-step reverse transcription-PCR Kit" for the production of cDNA
SCRIPTUM Standard is a mix for the convenient and rapid production of cDNA. The Kit produces a highly sensitive and specific reverse transcription followed by PCR in just one tube.
Due to the few pipetting steps the protocol is easy, convenient and quick to carry out in a single reaction tube. The SCRIPTUM standard reaches its high specificity by a genetically engineered reverse transcriptase with improved thermal stability. This leads to high yields of cDNA and at the same time very good results are also achieved for RNAs with a complex structure, and for long cDNA fragments.
The SCRIPTUM Standard kit contains all the necessary reagents for RT-PCR (reverse transcription followed by PCR) except template and primer. The handling in just one reaction tube and the few pipetting steps make the kit extremely comfortable and provides very fast excellent results.
Product | Article No. | Quantity |
---|---|---|
SCRIPTUM Standard | BS.49.020 | 50 reactions in 20 μl |
BS.49.100 | 250 reactions in 20 μl | |
BS.49.500 | 1250 reactions in 20 μl |
Protocols
The UNG Virus RNA Detection Kit is validated for the molecular diagnosis of SARS-CoV-2 which causes the new coronavirus infectious disease COVID-19.
It enables quantitative real-time analysis (QPCR) of RNA templates with double-labeled fluorescent probes.
Product | Order No. | Quantity |
---|---|---|
UNG Virus RNA Detection Kit | ||
BS.01.1250UNG | 2 x 1.25 ml | |
BS.01.6250UNG | 10 x 1.25 ml |
Protocol
UNG Virus RNA Detection Kit (One Tube RT-QPCR Mix)
Combination of 3 steps:
The Bio&SELL UNG Virus RNA Detection Kit combines UNG treatment, reverse transcription and the subsequent real time PCR in one tube. The combination of the steps in direct succession reduces the pipetting steps required to a minimum. This saves time and at the same time reduces the risk of contamination. All reagents required for the synthesis of high quality cDNA and quantitative real time PCR are included (except template, primer and probe).
1. UNG treatment:
In order to rule out contamination with other samples, the kit contains UNG (uracil-N-glycosylase), which breaks down all uracil residues from dU-containing DNA at the very beginning of reverse transcription and then becomes inactive when exposed to heat. On the other hand, the kit itself contains dUTP instead of dTTP, and the DNA amplified in this way can also be broken down by UNG in other reactions, thus preventing cross-contamination.
2. Reverse Transcription:
The ready-to-use enzyme mix contains a genetically optimized reverse transcriptase with improved thermal stability. This results in a higher specificity, larger cDNA yields and an improved transcription efficiency even with longer cDNA pieces with strong secondary structure formation.
3. Quantitative real-time analyzes (QPCR) with double-labeled fluorescent probes:
The real-time PCR technology based on double-labeled probes offers a highly sensitive and specific PCR system with multiplex capability. It requires two standard PCR primers and the DNA probe that hybridizes to an inner part of the amplicon. The sequence of the double-labeled DNA probe should preferably have no secondary structure and no primer-dimer formation.
The premium quality enzyme mix and the optimized reaction buffer with ultra pure dNTPs guarantee excellent amplification results.
For the isolation of viral RNA (also SARS-CoV-2) by column extraction, we recommend our Virus-RNA Isolation-Kit.