The fetal bovine serum is heated for 30 min. to 56° C in a water bath, whereby it is shaken several times. This treatment minimizes lot-to-lot variations in serum, because all thermolabile components are destroyed. Therefore, the results obtained in a serum-dependent cell culture system are more reproducible. Furthermore, the viral safety is enhanced, since heat-labile viruses are inactivated.
| Order No. | Quantity |
|---|---|
| FBS.HI.0100 | 100ml |
| FBS.HI.0500 | 500ml |
Application
Heat activated serum is recommended for:
- Measurements of lactate dehydrogenase in the culture supernatant as a marker for cell damage (serum LDH is destroyed by heat inactivation).
- Studies of vitamins and growth factors (are damaged wholly or partially, or have their concentrations reduced, by heat inactivation).
- Tests that do not tolerate presence of complement (complement destruction).