Bio&SELL 5x PCR Master Mix "ready-to-load" is a ready-mixed solution for PCR prepared for subsequent direct applications onto an agarose gel.
Please send me a 100ul sample PCR Master Mix ready to load for free!
| Component | Volume | Final Concentration |
|---|---|---|
| Bio&SELL 5x PCR-Mastermix |
10 µl | 1x |
| Primer 1 | x µl | 0.4 µM |
| Primer 2 | x µl | 0.4 µM |
| Template-DNA | x µl | 5-100 ng |
| Water | fill up to 50 µl |
The mix contains all reagents required for PCR, except DNA template, primers and water.
Includes blue and yellow dye for direct loading onto agarose gel.
Bio&SELL 5x PCR-Mastermix is optimized for PCR reactions with subsequent visualization of the products by agarose gel electrophoresis. The blue dye migrates in a 1% agarose gel with the same speed as 3.5 to 4.5 kb DNA fragments, the yellow dye roughly the same as the primer (<35-45 bp). Bio&SELL 5x PCR master mix contains Tween-20. If a detergent-free buffer system is required, please contact us in Germany at 49-9128-724 32 32.
The Bio&SELL 5x PCR master mix is not recommended for use with subsequent spectrophotometric measurement (absorbance or fluorescence measurements), since the two dyes can interfere with these applications.
| Article No. | Quantity |
|---|---|
| BS91.731.0100 | 100µl |
| 3 x 100µl | |
| BS91.731.1000 | 1000µl |
| 3 x 1000µl | |
| 5 x 1000µl | |
| 10 x 1000µl |
