Bio&SELL 5x PCR Master Mix "ready-to-load" is a ready-mixed solution for PCR prepared for subsequent direct applications onto an agarose gel.
|Primer 1||x µl||0.4 µM|
|Primer 2||x µl||0.4 µM|
|Template-DNA||x µl||5-100 ng|
|Water||fill up to 50 µl|
The mix contains all reagents required for PCR, except DNA template, primers and water.
Includes blue and yellow dye for direct loading onto agarose gel.
Bio&SELL 5x PCR-Mastermix is optimized for PCR reactions with subsequent visualization of the products by agarose gel electrophoresis. The blue dye migrates in a 1% agarose gel with the same speed as 3.5 to 4.5 kb DNA fragments, the yellow dye roughly the same as the primer (<35-45 bp). Bio&SELL 5x PCR master mix contains Tween-20. If a detergent-free buffer system is required, please contact us in Germany at 49-9128-724 32 32.
The Bio&SELL 5x PCR master mix is not recommended for use with subsequent spectrophotometric measurement (absorbance or fluorescence measurements), since the two dyes can interfere with these applications.
|Article No.||Quantity||Price in EURO|
|3 x 100µl||19.90 €|
|3 x 1000µl||169.90 €|
|5 x 1000µl||269.90 €|
|10 x 1000µl||484.00 €|