Bio&SELL 5x Multiplex QPCR Mixes for probe-based QRT-PCR (ROX or no ROX) are particularly optimized for multiplex quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x Multiplex QPCR Mix Rox contains optimized components, in particular Multiplex relevant buffer and Bio&SELL hot start Taq DNA polymerase in a proprietary buffer system that allows the detection up to 5-10 targets.
| Product | Article No. | Amount |
|---|---|---|
| 5x Multiplex QPCR Mix (No ROX) | BS76.525.0200 | 200µl = 50 reactions in 20 µl |
| BS76.525.1000 | 1000µl = 250 reactions in 20 µl | |
| BS76.525.5000 | 5000µl = 1.250 reactions in 20 µl |
Application
- detection and quantification of DNA and up to 10 cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
- Specific suitability for multiplex PCR reactions
Cycler and Probe Compatibility
Bio&SELL 5x Multiplex QPCR Mix (No ROX) is compatible with any probe system as well as real-time cyclers that do not require reference dye:
eg: Corbett Rotor-Gene ™ 3000, Rotor-Gene ™ 6000
e.g.: Eppendorf Mastercycler ® ep realplex
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | Final concentration |
|---|---|---|
| Bio&SELL 5x QPCR Mix (no ROX) |
4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |