Bio&SELL 5x Multiplex QPCR Mixes for probe-based QRT-PCR (ROX or no ROX) are particularly optimized for multiplex quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.

Bio&SELL 5x Multiplex QPCR Mix Rox contains optimized components, in particular Multiplex relevant buffer and Bio&SELL hot start Taq DNA polymerase in a proprietary buffer system that allows the detection of up to 5-10 targets.

ProductArticle No.AmountPrice in EURO
5x Multiplex QPCR Mix (No ROX) BS76.525.0200 200µl 39,00 €
BS76.525.1000 1000µl 174,00 €
BS76.525.5000 5000µl 779,00 €

Application

  • detection and quantification of DNA and cDNA targets
  • Profiling of gene expression
  • Microbial Detection
  • Determination of viral loads
  • Specific suitability for multiplex PCR reactions

Cycler and Probe Compatibility

Bio&SELL 5x Multiplex QPCR Mix (No ROX) is compatible with any probe system as well as real-time cyclers that do not require reference dye:
eg: Corbett Rotor-Gene ™ 3000, Rotor-Gene ™ 6000
e.g.: Eppendorf Mastercycler ® ep realplex

Recommended pipetting protocol (for probe-based kits)

ComponentVolumeFinal concentration
Bio&SELL 5x QPCR Mix
(no ROX)
4 µl 1 x
Primer 1 x µl 0,2 - 0,4 µM
Primer 2 x µl 0,2 - 0,4 µM
Sonde x µl 0,1 – 0,25 µM
Template-DNA x µl 1 – 50 ng
Water fill up to 20 µl