Bio&SELL 5x QPCR mixes for probe-based QRT-PCR (capillary) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix II (capillary) contains optimized components such as Bio&SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets."
Probe-based qRT-PCR
Product | Article No. | Aumont |
---|---|---|
Bio&SELL 5x QPCR Mix II (Capillary) | 76.540.0200 | 200µl = 50 reactions in 20 µl reaction volume |
76.540.1000 | 1000µl = 250 reactions in 20 µl reaction volume | |
76.540.5000 | 5000µl |
Application
- Detection and quantification of DNA and cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
Protocols
Cycler and Probe Compatibility
Bio & SELL QPCR Mix II (capillary) is compatible with the following Light-Cycler
eg: Roche LightCycler ® 1.x, LightCycler ® 2.0, LightCycler ® 480
Recommended pipetting protocol (for probe-based kits)
Component | Volume | Final concentration |
---|---|---|
Bio&SELL 5x QPCR Mix (Capillary) |
4 µl | 1 x |
Primer 1 | x µl | 0,2 - 0,4 µM |
Primer 2 | x µl | 0,2 - 0,4 µM |
Sonde | x µl | 0,1 – 0,25 µM |
Template-DNA | x µl | 1 – 50 ng |
Water | fill up to 20 µl |