Bio&SELL 5x QPCR mixes for probe-based QRT-PCR (capillary) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix II (capillary) contains optimized components such as Bio&SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets."

Probe-based qRT-PCR

ProductArticle No.AumontPrice in EURO
Bio&SELL 5x QPCR Mix II (Capillary) 76.540.0200 200µl = 50 reactions in 20 µl reaction volume 19.00 €
76.540.1000 1000µl = 250 reactions in 20 µl reaction volume 65.00 €
76.540.5000 5000µl 295.00 €

Application

  • Detection and quantification of DNA and cDNA targets
  • Profiling of gene expression
  • Microbial Detection
  • Determination of viral loads

Protocols

pdfdata sheet

Cycler and Probe Compatibility

Bio & SELL QPCR Mix II (capillary) is compatible with the following Light-Cycler
eg: Roche LightCycler ® 1.x, LightCycler ® 2.0, LightCycler ® 480

Recommended pipetting protocol (for probe-based kits)

ComponentVolumeFinal concentration
Bio&SELL 5x QPCR Mix
(Capillary)
4 µl 1 x
Primer 1 x µl 0,2 - 0,4 µM
Primer 2 x µl 0,2 - 0,4 µM
Sonde x µl 0,1 – 0,25 µM
Template-DNA x µl 1 – 50 ng
Water fill up to 20 µl