Bio&SELL 5x QPCR mixes for probe-based QRT-PCR (No ROX) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix II (No ROX) contains optimized components such as Bio& SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets".
Probe-based QRT-PCR
| Product | Article No. | Amount |
|---|---|---|
| Bio&SELL 5x QPCR Mix II (no ROX)) | 76.530.0200 | 200µl |
| 76.530.1000 | 1000µl | |
| 76.530.5000 | 5000µl |
Application
- detection and quantification of DNA and cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
Protocols
Cycler and Probe Compatibility
Bio&SELL QPCR Mix II (no ROX) is compatible with any probe system as well as real-time cyclers that do not require reference dye:
eg: Corbett Rotor-Gene ™ 3000, Rotor-Gene ™ 6000
e.g.: Eppendorf Mastercycler ® ep realplex
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | Final concentration |
|---|---|---|
| Bio&SELL 5x QPCR Mix (no ROX) |
4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |