Bio&SELL 5x QPCR Mixes for probe-based QRT-PCR (ROX or no ROX) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix ROX contains optimized components such as Bio&SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets."
|Product||Article No.||Amount||Price in EURO|
|5x QPCR Mix (ROX)||76.520.0200||200µl = 50 reactions in 20 µl reaction volume||17.00 €|
|76.520.1000||1000µl = 250 reaktions in 20 µl reaction volume||65.00 €|
|76.520.5000||5000µl = 1250 reaktions in 20 μl reaction volume||295.00 €|
- Detection and quantification of DNA and cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
Cycler and Probe Compatibility
Bio&SELL 5x QPCR Mix (ROX) is compatible with any probe system as well as real-time cyclers that require a high ROX concentration
e.g. ABI PRISM ® 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block).
Recommended pipetting protocol (for probe-based kits)
|Bio&SELL 5x QPCR Mix||4 µl||1 x|
|Primer 1||x µl||0,2 - 0,4 µM|
|Primer 2||x µl||0,2 - 0,4 µM|
|Sonde||x µl||0,1 – 0,25 µM|
|Template-DNA||x µl||1 – 50 ng|
|Water||fill up to 20 µl|
With internal reference dye ROX.
ROX is a passive internal reference dye used to normalize the fluorescent reporter signal during QPCR is on. The final concentration of ROX dye in the reaction mixture 500 nM.