Bio&SELL 5x QPCR Mixes for probe-based QRT-PCR (ROX or no ROX) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix ROX contains optimized components such as Bio&SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets."
Probe-based QRT-PCR
| Product | Article No. | Amount |
|---|---|---|
| 5x QPCR Mix (ROX) | 76.520.0200 | 200µl = 50 reactions in 20 µl reaction volume |
| 76.520.1000 | 1000µl = 250 reaktions in 20 µl reaction volume | |
| 76.520.5000 | 5000µl = 1250 reaktions in 20 μl reaction volume |
Application
- Detection and quantification of DNA and cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
Protocols
Cycler and Probe Compatibility
Bio&SELL 5x QPCR Mix (ROX) is compatible with any probe system as well as real-time cyclers that require a high ROX concentration
e.g. ABI PRISM ® 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block).
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | final concentration |
|---|---|---|
| Bio&SELL 5x QPCR Mix | 4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |
With internal reference dye ROX.
ROX is a passive internal reference dye used to normalize the fluorescent reporter signal during QPCR is on. The final concentration of ROX dye in the reaction mixture 500 nM.