qpcr Mastermixes

 The 5x QPCR Mixes EvaGreen® are optimized "ready-to-use" solutions for real-time applications such as quantitative PCR (QPCR), which require EvaGreen® or SYBR® Green as a dye.

They contain all necessary components for a quantitative PCR except template DNA and primers.

EvaGreen® dye - Advantages at a glance:

  • Lower PCR inhibition than SYBR®Green - can be used in higher concentrations
  • Higher sensitivity - signal stronger than SYBR®Green
  • Unrivaled high-resolution DNA melting curves
  • Thermostable - convenient handling
  • Not cytotoxic
  • Not mutagen
  • Suitable for all QPCR devices

Choose between 4 different QPCR mixes EvaGreen®:

  • Rox Mix: is compatible with all real-time cyclers that need Rox as an internal reference
    (Rox: passive, internal reference dye used to normalize the fluorescence reporter signal during the QPCR.).
  • No Rox Mix: is compatible with real-time cyclers that do not need a reference dye.
  • Capillary Mix: has been developed especially for real-time cycler, which work with capillaries and do not need a reference dye.
  • Robust Mix: due to specially designed buffer composition and GC enhancers the "ROBUST" mix is especially suitable for GC-rich templates and templates with long and frequent repeats. Also recommended for UNG treatments.

Application examples of the 5x QPCR mixes EvaGreen®

  • Detection and quantification of DNA and cDNA targets
  • Gene expression profiling
  • Microbial detection
  • Determination of viral loads
  • High resolution melting curve analysis (HRM)
  • UNG treatments (here we recommend the 5x QPCR Mix EvaGreen® (ROBUST))