Ribonuclease H (RNase H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA (RNA-DNA hybrids).

ProductOrder No.UnitsPrice in EURO
Bio&SELL Ribonuclease H  BS.RNase-H-100  100  199,-
 BS.RNase-H-250  250  469,-
 larger quantities    on request

Applications

  • Degradation of mRNA - prior to synthesis of second strand cDNA
  • Degradation of RNA after first strand cDNA synthesis - RT-PCR and RT-QPCR
The RNAse H can be inactivated by heating at 65 °C for 10 min.

Protocol

pdfData sheet