Ribonuclease H (RNase H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA (RNA-DNA hybrids).
| Product | Order No. | Units |
|---|---|---|
| Bio&SELL Ribonuclease H | BS.RNase-H-100 | 100 |
| BS.RNase-H-250 | 250 | |
| larger quantities |
Applications
- Degradation of mRNA - prior to synthesis of second strand cDNA
- Degradation of RNA after first strand cDNA synthesis - RT-PCR and RT-QPCR
The RNAse H can be inactivated by heating at 65 °C for 10 min.
