Plant RNA Kit
Our Plant RNA Kit is ideal for the fast and efficient isolation of RNA from fresh, dried or frozen plant materials of all kinds (e.g. leaves, stems, flowers, seeds, roots etc.). Even challenging starting materials can be split with the help of two specially developed lysis buffers. Depending on the amount and type of your starting tissue, you can extract up to 70-100 µg of RNA in just 30 minutes (after homogenization of the starting material). Our Plant RNA Kit has an excellent RNA binding capacity of 100 µg RNA (depending on the type and amount of starting material) and allows fast and effective RNA isolation of the highest quality without the use of toxic substances such as beta-mercaptoethanol.
Properties
Universal use - Suitable for fresh, dried or frozen plant material
(e.g. leaves, stems, flowers, seeds, roots, fruits)
High yield - Binding capacity: 100 µg RNA (depending on type and amount of starting material)
Fast isolation - In only 15-30 min (after homogenization of the material)
Easy handling - Column-based RNA isolation
Good throughput - Suitable for up to 100 mg tissue
High safety - Without the use of toxic substances such as beta-mercaptoethanol
Good to know
Since plants have both primary and secondary plant substances, the isolation of RNA from plant tissues is often very difficult. Primary plant substances (carbohydrates, proteins and fats) are the main components of the plant. Secondary plant substances (colorants, fragrances and flavorings) enable the plant to attract insects and ward off pests. Secondary plant substances are, for example, polyphenols (such as flavonoids and anthocyanins), carotenoids or alkaloids. RNA isolation from plant tissues often leads to interference of the RNA with polyphenols, polysaccharides and lipids or even to degradation of the RNA. This reduces the RNA yield and lowers the quality of the extracted RNA.
Our Plant RNA Kit is optimized for RNA isolation from plant material. With two specially developed lysis buffers, our kit enables fast and efficient RNA isolation from the most challenging plant materials. Non-lysed plant parts and plant DNA are filtered off using an additional column and plant inhibitors and metabolites are removed. Elution is carried out with RNase-free water (some elution buffers distort photometric concentration determinations). We recommend an elution volume of at least 20 µl (better: 30 - 80 µl). After homogenization, your samples can also be stored in lysis buffer (at -20 °C) for a longer period of time without any concerns.
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