RNA Mini Kit
Our RNA Mini Kit has been specially developed for the isolation of total RNA from tissue samples, cells, bacteria and biopsies. Our column-based kit contains a lysis buffer that is optimized for the efficient digestion of different starting materials and simultaneously inhibits RNases. Integrated DNA filtering allows isolation of total RNA without DNA digestion in only 15-30 min. Our RNA Mini Kit has an excellent RNA binding capacity of approx. 100 µg and allows fast and effective isolation of total RNA of highest quality (ratio A260 / A280: 1.7 - 2.0).
Properties
Fast isolation - in only 15 - 30 min
Easy handling - Column-based RNA isolation
High yield - Binding capacity approx. 100 µg RNA (depending on quantity/quality of sample material)
Good throughput - Suitable for up to 5 x 106 cells or for tissue samples up to 20 mg
High purity - A260/A280 ratio: 1.7 - 2.0
High safety - Without the use of toxic substances such as phenol, DTT, beta-mercaptoethanol
Good to know
Original RNA purification methods were based on a two-phase extraction followed by alcohol precipitation of the RNA (e.g. phenol-chloroform-isoamyl alcohol extraction). These methods are time-consuming and dangerous due to the use of toxic substances such as phenol. In addition, short RNA molecules are only obtained with a low yield. Our RNA Mini Kit is based on the adsorption of RNA on silicate columns and offers you a quick, simple and safe alternative.
To make your RNA isolation even more efficient we have the following tips:
1. Inactivation of RNases
The biggest challenge in RNA isolation is posed by RNases. RNases digest RNA non-specifically and are ubiquitous in cells and tissues. Therefore, ensure an RNase-free laboratory environment and inactivate the RNases directly during sample preparation (by using e.g. RNaseStop, article no.: BS.RS.1000). Mercaptoethanol or dithiothreitol is often added to the lysis buffer to inactivate RNases by cleaving their disulfide bridges. However, both substances are harmful to health. Our RNA Mini Kit offers a safe alternative. The lysis buffer of our RNA Mini Kit is not only optimized for effective cell disruption, but also for the inhibition of RNases without the use of toxic substances.
2. Amount of sample material
The quantity and quality of the starting material is also crucial for successful RNA isolation. Too much or contaminated sample material reduces the yield and purity due to incomplete cell lysis or clumping in the column. For optimal results with our RNA Mini Kit, use up to 20 mg tissue, 5 x 106 cells or up to 1 × 109 bacteria. Also make sure that your samples are fully lysed and homogenized before placing them on the columns for optimal yields.
3. Removal of DNA contamination
Another problem with RNA purification is DNA contamination, which reduces the purity of the RNA. Conventional RNA isolation methods remove DNA through additional, lengthy DNase digestion. Our RNA Mini Kit effectively removes DNA through integrated DNA filtering, saving you time for other experiments or a cup of coffee.