RNA Isolation Tri-Liquid
RNA Isolation Tri-Liquid is a reagent for the simple and efficient isolation of total RNA from different starting materials (tissue, cells, bacteria, viruses, plants, etc.). Extraction is carried out using a particularly time-saving one-step liquid-phase separation. With this method, RNA can be efficiently extracted from limited starting material with small tissue quantities and cell numbers (5 x 106) as well as from large quantities (tissue up to 100 mg and cells > 107).
Properties
Fast - preparation time: approx. 1 hour
Efficient - for both limited starting material and large quantities
Reliable - Extraction of high-quality, undegraded RNA
Reproducible - Free from DNA and protein contamination
Good to know
RNA Isolation Tri-Liquid contains a mixture of phenol and guanidinisothiocyanate in a monophasic solution. After the addition of chloroform and subsequent centrifugation, the homogenate separates into three phases, a colored lower organic phase, a whitish interphase and an upper colorless aqueous phase. The RNA is located in the upper aqueous phase. The RNA is precipitated from this aqueous phase by adding alcohol.
RNA extraction with RNA Isolation Tri-Liquid can be performed in approx. 1 hour. The RNA extracted is ungraded and of high quality. It can be used for a variety of downstream applications, such as Northern analyses, cDNA synthesis, RT-PCR reactions, dot-blot hybridizations, poly(A)+ selections, in vitro translations, cloning and RNase assays.