si-/miRNA Kit

Our optimized si-/miRNA kit enables the rapid and highly efficient isolation of the smallest RNA molecules from tissue samples, eukaryotic cells, biopsies and bacteria. With integrated DNA filtering, our kit allows RNA isolation without DNAse digestion in only 15-40 min. Optimized silica columns are used together with specially developed buffer solutions (without phenol, DTT or beta-mercaptoethanol) that are as harmless to health as possible. Our kit achieves an excellent RNA binding capacity of approx. 100 µg and highest purity (ratio A260/A280: 1.7 - 2.0) of your RNA. It is ideal for the analysis of miRNAs and siRNAs as well as for the efficient isolation of total RNA with small RNA sequences.

Fast isolation - in only 15 - 40 min
High yield - Binding capacity approx. 100 µg RNA (depending on quantity/quality of starting material)
Good throughput - Suitable for up to 5 x 106 cells or for tissue samples up to 20 mg
High purity - A260/A280 ratio: 1.7 - 2.0
High safety - Without the use of toxic substances such as phenol, chloroform, beta-mercaptoethanol

Natural siRNAs and miRNAs are highly conserved, non-coding ribonucleic acids consisting of 20-25 nucleotides. The two types of RNA differ mainly in their origin.

MiRNAs are single-stranded and are encoded by pri-miRNA genes. They play an important role in gene regulation, especially in cellular gene silencing. MiRNAs are complementary to an information-carrying messenger RNA (mRNA) and block its protein biosynthesis by binding to it. The bound mRNA can no longer serve as a template for translation. Thus, miRNAs regulate gene expression in a highly specific manner at the post-transcriptional level. MiRNAs can therefore also contribute to the development of diseases such as cancer or metabolic disorders.

SiRNAs are produced by the cleavage of a long double-stranded RNA and enter the cell primarily from the outside (e.g. through RNA viruses). In the case of a virus infection, they are formed as cleavage products during the replication of the virus RNA and serve the cell to combat the intruder by means of gene silencing. Through RNA interference, they initiate the cleavage of the mRNA or prevent the attachment of ribosomes, which also suppresses protein biosynthesis. Synthetic siRNAs are often used in basic research for gene knockout experiments.

Use our optimized si-/miRNA kit to conveniently and effectively isolate your desired siRNAs and miRNAs!