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RT-qPCR Probe No Rox

RT-qPCR Probe No Rox has been specially optimized for the quantitative, real-time analysis of RNA templates using double-labelled probes (e.g. TaqMan® probes). Our kit combines the reverse transcription (RT) of template RNAs with a subsequent quantitative real-time polymerase chain reaction (qPCR) in a single reaction tube (one-step). The One-Step-Mix thus achieves the simplest feasibility, time savings and a reduced risk of contamination, which contributes to maximum reproducibility of your experiments. RT-qPCR Probe No Rox is suitable for the absolute quantification of RNA (also for GC- and AT-rich RNA templates), for relative gene expression analyses and for the detection of low copy genes.

RT-qPCR Probe No Rox contains a thermostable, highly active M MuL V reverse transcriptase in combination with an RNase inhibitor, which allows efficient cDNA synthesis. A highly sensitive hot-start Taq polymerase achieves high sensitivity and specificity under standard qPCR as well as under very fast qPCR conditions.

Our kit contains all necessary reagents for the synthesis of high quality cDNA and quantitative, real time PCR (except RNA template, primer and dye-labeled probe). This kit does not contain the reference dye Rox.

Article list

ProductsArticle numberQuantityShop
RT-qPCR Probe No Rox
BS53.0200
250 reactions à 20µl
BS53.1000
1250 reactions à 20µl

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