Scriptum reverse transcriptases

Bio&SELL SCRIPTUM enzyme mixes for the synthesis of cDNA

The Bio&SELL Scriptum reverse transcriptase-sets at a glance:

The Bio&SELL Scriptum reverse transcriptases are recombinantly produced versions of the M-MLV reverse transcriptase, in which the RNase H activity is eliminated and the "thermal stability" has been improved.

These modifications result in:

  • increased specificity
  • higher cDNA yields and
  • an improved effectiveness in the synthesis of full-length cDNA synthesis up to 14 kb.

The simple handling of the kits, their high reproducibility and the fast protocols ultimately lead to a high quality cDNA perfectly fitted for further applications (such as a QPCR).

Choose between 3 different reverse transcriptases:

Scriptum-37 reverse transcriptase:

  • For the synthesis of cDNA molecules from 100 bp to 7 kb in length
  • Easy to fit protocol: Supplied along with the enzyme Scriptum-37 reverse transcriptase, 2 buffer solutions, a MgCl2 solution and a DTT stock solution are included.
  • Particularly suitable for routine applications with simple templates
  • Temperature optimum of this reverse transcriptase is 37 °C

Scriptum-55 reverse transcriptase:

  • for the synthesis of cDNA molecules from 100 bp to 10 kb in length
  • easy to fit protocol: Supplied along with the enzyme Scriptum-55 reverse transcriptase, 2 buffer solutions, MgCl2 solution and a DTT stock solution are included
  • for simple and difficult templates
  • optimum temperature for the synthesis of longer cDNA fragments (> 7kb) is 50 °C
  • possible synthesis temperature up to 55 °C

Scriptum-55 reverse transcriptase "improved":

  • for the synthesis of cDNA molecules from 100 bp to 14 kb in length
  • enzyme latest generation: specifically designed for difficult templates (strong secondary structures of the RNA, RNA loops, long RNAs)
  • increased thermal stability: working temperature of Scriptum-55 reverse transcriptase "improved" is up to 55 °C (important for the synthesis of long cDNA fragments> 7kb and RNAs with strong secondary structures)
  • highly sensitive and highly processive! 

Image: © Gernot Krautberger - Fotolia.com