Bio&SELL offers a versatile range of RNA products for molecular biology research. The products are specifically designed for RNA purification and analysis, as well as cDNA synthesis. The portfolio includes RNA isolation kits, RT-PCR kits, RT-qPCR one-step mixes, reverse transcriptases, and reagents for RNase inhibition.
RNA Isolation Kits for Various Source Materials
Efficient RNA isolation is the most important prerequisite for numerous molecular biology applications. Each source material poses different challenges for effective RNA extraction and purification.
Bio&SELL offers column-based RNA isolation kits for a variety of biological source materials—ranging from cells and tissues to blood and plant material, all the way to viral nucleic acids:
The RNA isolation product line is complemented by the liquid reagent RNA Tri-liquid, which extracts RNA using a particularly time-saving one-step liquid-phase separation.
RT-PCR Kits for the Reverse Transcription of RNA
In reverse transcriptase PCR (RT-PCR), RNA is first transcribed into complementary DNA (cDNA). This cDNA can then be amplified using PCR and used for further molecular biological analyses.
Bio&SELL’s RT-PCR kits contain perfectly matched reagents for cDNA synthesis and subsequent applications. With products such as Scriptum High Precise and Scriptum First Strand, high-performance, ready-to-use kits are available to meet a variety of RT-PCR requirements.
RT-PCR is used in numerous research fields, for example, in the analysis of RNA transcripts and as a preparatory step for further analyses.
RT-qPCR One-Step Mixes for Quantitative RNA Analysis
RT-qPCR (Reverse Transcription Quantitative PCR) combines reverse transcription (RT) of RNA with quantitative real-time PCR (qPCR). In a one-step procedure, reverse transcription and qPCR take place within a single reaction mixture. This simplifies workflows and reduces the need for additional pipetting steps.
Bio&SELL offers RT-qPCR one-step mixes for various detection methods. These include RT-qPCR SYBR® Green Rox and RT-qPCR Probe Rox.
These reagents are suitable for molecular biology research applications in which RNA is to be analyzed and quantified using RT-qPCR.
Reverse Transcriptases for cDNA Synthesis
Reverse transcriptases are RNA-dependent DNA polymerases that enable the synthesis of complementary DNA from an RNA template. They are therefore among the key enzymes used in numerous methods of RNA analysis.
Bio&SELL offers a variety of reverse transcriptases for molecular biology research:
The selection of a suitable reverse transcriptase depends on the RNA template, reaction conditions, and intended application, and allows for the tailored adaptation of reverse transcription to the specific experimental workflow.
RNase Inhibitors for Protecting Sensitive RNA
RNA is particularly sensitive to degradation by ribonucleases (RNases). Even low levels of RNase contamination can compromise the quality of RNA samples and negatively impact subsequent analyses.
RNase inhibition reagents can be used to protect RNA from enzymatic degradation during sensitive steps. Bio&SELL offers, among other products, an RNase inhibitor and RNaseSTOP for this purpose.
The use of suitable RNase inhibitors helps protect the RNA and contributes to maintaining the integrity of the starting material for subsequent molecular biology applications.
RNA Products for Reliable Molecular Biology Workflows
From RNA isolation and reverse transcription to RT-PCR and RT-qPCR, Bio&SELL’s RNA portfolio includes products for key steps in molecular biology research.
Whether it's an RNA isolation kit, RT-PCR kit, RT-qPCR one-step mix, reverse transcriptase, or RNase inhibitor - discover Bio&SELL's RNA products and choose the right reagents for your specific molecular biology workflow.
FAQs
Which RNA isolation kit is right for my sample?
The kit you need to isolate your RNA depends on the source material. Bio&SELL offers kits for various source materials, such as cells, tissue, blood, plant material, and viral nucleic acids. We also offer a separate kit for small RNAs, such as siRNA or miRNA.
What is the difference between an RNA isolation kit and RNA Tri-Liquid?
RNA isolation kits enable the purification of RNA from various source materials. RNA Tri-Liquid is a liquid reagent for RNA extraction using a single-step liquid-phase separation. Which method is suitable depends, among other things, on the sample and the workflow.
