Scriptum First strand

The SCRIPTUM FIRST cDNA synthesis kit includes all reagents needed for the first strand cDNA synthesis, and combines ease of use with high flexibility.

A reverse transcriptase in premium quality, ultra-pure dNTPs and an optimized reaction buffer ensure superior results with highest reproducibility. The kit is optimized for high efficiency for a wide range of primer-template combinations. The SCRIPTUM FIRST reverse transcriptase is a recombinantly produced version of the M-MLV reverse transcriptase (M-MLV RT), wherein the eliminated RNase H activity and the thermal stability was improved.

Application

Synthesis of highly structured and long cDNA fragments, extremely sensitive and highly specific RT PCR, DNA labeling. The kit contains both so-called random hexamer primers and oligo (dT) 20 primer. Optionally, gene-specific primers can be used. Here, however, we recommand using the "Scriptum high precision " as an alternative cDNA synthesis kit ".

Protocols