UNG virus RNA detection kit

The UNG Virus RNA Detection Kit is validated for the molecular diagnosis of SARS-CoV-2 which causes the new coronavirus infectious disease COVID-19.

It enables quantitative real-time analysis (QPCR) of RNA templates with double-labeled fluorescent probes.

Protocol

Data sheet

UNG Virus RNA Detection Kit (One Tube RT-QPCR Mix)

Combination of 3 steps:

The Bio&SELL UNG Virus RNA Detection Kit combines UNG treatment, reverse transcription and the subsequent real time PCR in one tube. The combination of the steps in direct succession reduces the pipetting steps required to a minimum. This saves time and at the same time reduces the risk of contamination. All reagents required for the synthesis of high quality cDNA and quantitative real time PCR are included (except template, primer and probe).

1. UNG treatment:
In order to rule out contamination with other samples, the kit contains UNG (uracil-N-glycosylase), which breaks down all uracil residues from dU-containing DNA at the very beginning of reverse transcription and then becomes inactive when exposed to heat. On the other hand, the kit itself contains dUTP instead of dTTP, and the DNA amplified in this way can also be broken down by UNG in other reactions, thus preventing cross-contamination.

2. Reverse Transcription:

The ready-to-use enzyme mix contains a genetically optimized reverse transcriptase with improved thermal stability. This results in a higher specificity, larger cDNA yields and an improved transcription efficiency even with longer cDNA pieces with strong secondary structure formation.

3. Quantitative real-time analyzes (QPCR) with double-labeled fluorescent probes:

The real-time PCR technology based on double-labeled probes offers a highly sensitive and specific PCR system with multiplex capability. It requires two standard PCR primers and the DNA probe that hybridizes to an inner part of the amplicon. The sequence of the double-labeled DNA probe should preferably have no secondary structure and no primer-dimer formation.

The premium quality enzyme mix and the optimized reaction buffer with ultra pure dNTPs guarantee excellent amplification results.

For the isolation of viral RNA (also SARS-CoV-2) by column extraction, we recommend our Virus-RNA Isolation-Kit.