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Bio&SELL 5x Multiplex QPCR Mixes for probe-based QRT-PCR (ROX or no ROX) are particularly optimized for multiplex quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x Multiplex QPCR Mix Rox contains optimized components, in particular Multiplex relevant buffer and Bio&SELL hot start Taq DNA polymerase in a proprietary buffer system that allows the detection up to 5-10 targets.
| Product | Article No. | Amount |
|---|---|---|
| 5x Multiplex QPCR Mix (No ROX) | BS76.525.0200 | 200µl = 50 reactions in 20 µl |
| BS76.525.1000 | 1000µl = 250 reactions in 20 µl | |
| BS76.525.5000 | 5000µl = 1.250 reactions in 20 µl |
Application
- detection and quantification of DNA and up to 10 cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
- Specific suitability for multiplex PCR reactions
Cycler and Probe Compatibility
Bio&SELL 5x Multiplex QPCR Mix (No ROX) is compatible with any probe system as well as real-time cyclers that do not require reference dye:
eg: Corbett Rotor-Gene ™ 3000, Rotor-Gene ™ 6000
e.g.: Eppendorf Mastercycler ® ep realplex
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | Final concentration |
|---|---|---|
| Bio&SELL 5x QPCR Mix (no ROX) |
4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |
Bio&SELL 5x Multiplex QPCR Mixes for probe-based QRT-PCR (ROX or No ROX) are particularly optimized for multiplex quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x Multiplex QPCR Mix Rox contains optimized components, in particular Multiplex relevant buffer and Bio&SELL Hot Start Taq DNA polymerase in a proprietary buffer system that allows the detection of up to 5-10 targets.
| Product | Article No. | Amount |
|---|---|---|
| 5x Multiplex QPCR Mix (ROX) | BS76.515.0200 | 200µl = 50 reactions in 20 µl reaction volume |
| BS76.515.1000 | 1000µl = 250 reaktions in 20 µl reaction volume | |
| BS76.515.5000 | 5000µl = 1250 reaktions in 20 μl reaction volume |
Application
- Detection and quantification of DNA and up to 10 cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
- Specific suitability for multiplex PCR reactions
Cycler and Probe Compatibility
Bio&SELL 5x QPCR Mix (ROX) is compatible with any probe system as well as real-time cyclers that require a high ROX concentration
e.g. ABI PRISM ® 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block).
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | final concentration |
|---|---|---|
| 5x Multiplex QPCR Mix | 4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |
With internal reference dye ROX.
ROX is a passive internal reference dye used to normalize the fluorescent reporter signal during QPCR is on. The final concentration of ROX dye in the reaction mixture 500 nM.
Protocol
Bio&SELL 5x QPCR mixes for probe-based QRT-PCR (No ROX) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix II (No ROX) contains optimized components such as Bio& SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets".
Probe-based QRT-PCR
| Product | Article No. | Amount |
|---|---|---|
| Bio&SELL 5x QPCR Mix II (no ROX)) | 76.530.0200 | 200µl |
| 76.530.1000 | 1000µl | |
| 76.530.5000 | 5000µl |
Application
- detection and quantification of DNA and cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
Protocols
Cycler and Probe Compatibility
Bio&SELL QPCR Mix II (no ROX) is compatible with any probe system as well as real-time cyclers that do not require reference dye:
eg: Corbett Rotor-Gene ™ 3000, Rotor-Gene ™ 6000
e.g.: Eppendorf Mastercycler ® ep realplex
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | Final concentration |
|---|---|---|
| Bio&SELL 5x QPCR Mix (no ROX) |
4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |
Bio&SELL 5x QPCR mixes for probe-based QRT-PCR (capillary) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix II (capillary) contains optimized components such as Bio&SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets."
Probe-based qRT-PCR
| Product | Article No. | Aumont |
|---|---|---|
| Bio&SELL 5x QPCR Mix II (Capillary) | 76.540.0200 | 200µl = 50 reactions in 20 µl reaction volume |
| 76.540.1000 | 1000µl = 250 reactions in 20 µl reaction volume | |
| 76.540.5000 | 5000µl |
Application
- Detection and quantification of DNA and cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
Protocols
Cycler and Probe Compatibility
Bio & SELL QPCR Mix II (capillary) is compatible with the following Light-Cycler
eg: Roche LightCycler ® 1.x, LightCycler ® 2.0, LightCycler ® 480
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | Final concentration |
|---|---|---|
| Bio&SELL 5x QPCR Mix (Capillary) |
4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |
Bio&SELL 5x QPCR Mixes for probe-based QRT-PCR (ROX or no ROX) are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.
Bio&SELL 5x QPCR Mix ROX contains optimized components such as Bio&SELL Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets."
Probe-based QRT-PCR
| Product | Article No. | Amount |
|---|---|---|
| 5x QPCR Mix (ROX) | 76.520.0200 | 200µl = 50 reactions in 20 µl reaction volume |
| 76.520.1000 | 1000µl = 250 reaktions in 20 µl reaction volume | |
| 76.520.5000 | 5000µl = 1250 reaktions in 20 μl reaction volume |
Application
- Detection and quantification of DNA and cDNA targets
- Profiling of gene expression
- Microbial Detection
- Determination of viral loads
Protocols
Cycler and Probe Compatibility
Bio&SELL 5x QPCR Mix (ROX) is compatible with any probe system as well as real-time cyclers that require a high ROX concentration
e.g. ABI PRISM ® 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block).
Recommended pipetting protocol (for probe-based kits)
| Component | Volume | final concentration |
|---|---|---|
| Bio&SELL 5x QPCR Mix | 4 µl | 1 x |
| Primer 1 | x µl | 0,2 - 0,4 µM |
| Primer 2 | x µl | 0,2 - 0,4 µM |
| Sonde | x µl | 0,1 – 0,25 µM |
| Template-DNA | x µl | 1 – 50 ng |
| Water | fill up to 20 µl |
With internal reference dye ROX.
ROX is a passive internal reference dye used to normalize the fluorescent reporter signal during QPCR is on. The final concentration of ROX dye in the reaction mixture 500 nM.
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