Bio&SELL 5x QPCR Mixes EvaGreen®(ROX), for EvaGreen®-based QRT-PCR are optimal for real-time applications such as quantitative PCR and contain all necessary components for quantitative PCR except template DNA and primers.

EvaGreen-based QRT-PCR
EvaGreen® is compatible with all common real-time PCR cyclers - simply select the default setting for SYBR® Green or FAM!

 

ProductArticel No.QuantityPrice in EURO
5xQPCR Mix EvaGreen® (ROX) 76.580.0200 200µl = 50 reactions in 20 µl reaction volume 24.90 €
76.580.1000 1000µl = 250 reactions in 20 µl reaction volume 119.00 €
76.580.5000 5000µl = 1250 reactions in 20 μl reaction volume 535.00 €

Application

  • Detection and quantification of DNA und cDNA targets
  • Profiling of gene expression
  • Microbial Detectionn
  • Determination of viral load

Protocols

pdfdata sheet

Cycler and Probe Compatibility

Bio&SELL 5x QPCR Mix EvaGreen® (ROX) is compatible with real-time cyclers that require a high ROX concentration

e.g. ABI PRISM® 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block).

Recommended pipetting protocol (for probe-based kits)

ComponentVolumeFinalconcentration
Bio&SELL 5x QPCR
Mixe EvaGreen® (ROX)
4 µl 1 x
Primer 1 x µl 0,2 - 0,4 µM
Primer 2 x µl 0,2 - 0,4 µM
Template-DNA x µl 1 – 50 ng
Water fill up to 20 µl  

With internal reference dye ROX.
ROX is a passive internal reference dye that is used for normalization of the fluorescent reporter signal during QPCR.
The final concentration of ROX dye in the reaction mixture is 500 nM.

* EvaGreen® is a DNA-binding dye with many advantages over SYBR ® Green I in quantitative PCR. Although on a similar spectrum, EvaGreen ® has the following important advantages over SYBR ® Green I : EvaGreen ® has a lower PCR inhibition; is an extremely stable dye and is neither mutagenic nor cytotoxic. EvaGreen ® is compatible with all common real-time PCR cyclers - simply select the default setting for SYBR ® Green or FAM!

Sales are exclusively with our partner Bio-Budget Technologies.

This product is provided under an agreement with BIOTIUM, INC., and the manufacture, use, sale or import of this product is subject to one or more of pending patent applications owned by BIOTIUM and ALLELOGIC. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact BIOTIUM, INC., Business Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel: 510-265-1027, Fax: 510-265-1352.
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