Bio&SELL 5x Multiplex QPCR Mixes for probe-based QRT-PCR (ROX or No ROX) are particularly optimized for multiplex quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.

Bio&SELL 5x Multiplex QPCR Mix Rox contains optimized components, in particular Multiplex relevant buffer and Bio&SELL Hot Start Taq DNA polymerase in a proprietary buffer system that allows the detection of up to 5-10 targets.

 

ProductArticle No.AmountPrice in €
5x Multiplex QPCR Mix (ROX) BS76.515.0200 200µl = 50 reactions in 20 µl reaction volume 39.00 €
BS76.515.1000 1000µl = 250 reaktions in 20 µl reaction volume 174.00 €
BS76.515.5000 5000µl = 1250 reaktions in 20 μl reaction volume 779.00 €

Application

  • Detection and quantification of DNA and up to 10 cDNA targets
  • Profiling of gene expression
  • Microbial Detection
  • Determination of viral loads
  • Specific suitability for multiplex PCR reactions

Cycler and Probe Compatibility

Bio&SELL 5x QPCR Mix (ROX) is compatible with any probe system as well as real-time cyclers that require a high ROX concentration
e.g. ABI PRISM ® 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block).

Recommended pipetting protocol (for probe-based kits)

ComponentVolumefinal concentration
5x Multiplex QPCR Mix 4 µl 1 x
Primer 1 x µl 0,2 - 0,4 µM
Primer 2 x µl 0,2 - 0,4 µM
Sonde x µl 0,1 – 0,25 µM
Template-DNA x µl 1 – 50 ng
Water fill up to 20 µl  

With internal reference dye ROX.
ROX is a passive internal reference dye used to normalize the fluorescent reporter signal during QPCR is on. The final concentration of ROX dye in the reaction mixture 500 nM.

 

Protocol

pdf 5x Multiplex QPCR Mix