Bio&SELL RNA Tri-Liquid is a reagent for the efficient isolation of total RNA from different starting materials (tissues, cells, bacteria, plants, etc.) in varying amounts. The extraction method is based on a particularly time-saving One-step Liquid-Phase Separation.
Bio&SELL RNA Tri-Liquid contains a mixture of phenol and guanidine isothiocyanate in a monophasic solution. After the addition of chloroform, followed by centrifugation, the homogenate separates into three phases, a colored lower organic phase, a whitish interphase and an upper colorless aqueous phase. The RNA is located in the upper aqueous phase. From this aqueous phase, the RNA is precipitated by the addition of alcohol (see Figure below).
The RNA- Extraction with Bio&SELL RNA Tri-Liquid can be performed in about 1 hour. The RNA extracted here is not degraded and in high quality condition. It can be used for a variety of downstream applications, such as Northern analysis, cDNA synthesis, RT-PCR reactions, Dot Blot hybridizations, poly (A) + selection, in vitro translation, cloning and RNase assays.
|Bio&SELL RNA Tri-Liquid||BS67.211.0100||1 x 100ml|
|3 x 100ml|
|5 x 100ml|