Bio&SELL Hot Start Taq DNA-Polymerase - reliable, flexible and affordable.

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Optimize your PCR results with the Hot-Start Taq-DNA-polymerase

The Bio&SELL Hot-Start Taq-DNA-polymerase has evolved from our time-tested Taq-DNA-Polymerase.
Due to a modification, the enzyme activity of the Bio&SELL Hot-Start Taq-DNA-Polymerase is released after only a single 15- minute incubation at 95 ° C.
This will bring significant benefits:

  • pipetting without time pressure
  • no background amplification due to nonspecific annealing of primers
  • no interference from primer dimers or other unwanted secondary structure formation

Each PCR is special - with the Bio&SELL Hot-Start Taq-DNA-Polymerase each of your PCR reactions can be individually optimized.
Many extras are included:

  • three different buffer systems with and without detergent
  • separate MgCl2 solution
  • S-solution for GC-rich templates and minimizing background

 

Article No.UnitsPrice in EURO
BS91.771.0100 100 52,- €
BS91.771.0500 500 175,- €
  3 x 500 510,- €
  5 x 500 740,- €
  >11 x 500 on request

 

 

Protocols

Concentration: 5units/µl                                         

Packaging sizes:  100µl - 500µl

Shipping temperature: at room temperature

Storagetemperatur:  -20° Celsius

 

Have you already optimized your reaction?

Now you can work even more efficiently with the Hot-Start Taq-DNA-Polymerase Mix from Bio&SELL: all three buffer systems are also available as Hot-Start Taq Polymerase Mix.

Source

original form is the thermostable DNA Polymersase, which was isolated from the thermophilic bacterium Thermus aquaticus. In the present enzyme is a modified variant, which was recombinantly expressed in E. coli. Hot-start Taq DNA polymerase is activated by incubation at 95 ° over a period of 15 minutes. The prevention of nonspecific binding of primers and the formation of primer dimers at low temperatures at the beginning of the PCR is hereby possible.

Application and Quality Control

Primer extension reaction: the enzyme is free of nicking and priming activities, exonucleases and unspecific endonucleases. SDS / PAGE: 95 kD band, purity> k98%. Activity and stability were tested by PCR. The error rate per cycle per Nukeotid is 8.3x10-5 1.2x104 accuracy. The half-life at 95 °C is 90 min.

Storage and dilution buffer: 50% glycerol (v / v), 20 mM Tris • HCI (pH 8.7 at 25 ° C), 100 mM KCl, 0.1 mM EDTA and stabilizers.

Unit definition

One unit is the amount of enzyme required to 10 nmol of dNTP in 30 min converting at 74 ° C in an acid-insoluble form.