The Bio&SELL S-Dis hotstart polymerase is a thermostable polymerase with a strong strand displacement activity.
The S-Dis hotstart polymerase achieves excellent results in all methods of DNA-Amplification (LAMP, PCR, PCDR).
Because unlike the naturally occurring enzymes with a strong strand displacement activity (Bst or Phi29 - polymerase) that are active only until 68 °C, this novel polymerase is stable up to 93 °C.
The activity of the S-Dis hotstart polymerase is blocked by an antibody. Only by heating above 90 ° C, the antibody is inactivated and the reaction starts.
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PCDR (Polymerase Chain Reaction Displacement)
The PCDR offers a much higher efficiency and sensitivity than conventional PCR methods. The S-Dis polymerase is the enzyme of choice to optimize the PCDR. For within the PCDR the enzyme combines his two unique advantages: the strand displacement activity and high thermal stability.
The strand displacement activity of S-Dis polymerase is temperature-dependent; with an optimum for LAMP applications between 62-68 ° C. Due to the high thermal stability of the enzyme, an initial DNA denaturation step of the LAMP is carried out (92 ° C for 2 min), which greatly improves the reaction. The LAMP can be carried out with 15 - 50 Units S -Dis polymerase per 50 μl reaction volume.
The S-Dis polymerase is suitable for the amplification of short (from 100 bp) and very long (up to 20-30 kb) DNA fragments of all kinds. From simple (plasmids) to more complex templates (genomic DNA) no special optimization is necessary. Compared to a conventional Taq - Polymerase improved productivity is achieved through the use of S-Dis polymerase in PCR applications: more yield, higher speed, better efficiency. Even "single copy" - templates are amplified with very good results.