Bio&SELL Taq-Polymerase - robust, versatile and economical
The Bio&SELL Taq-DNA-Polymerase achieves excellent results in your PCR applications because you can optimize each of your PCR reactions individually.
Included in the delivery of Bio&SELL Taq Polymerase:
- Two different buffer systems with and without detergent
- Separate MgCl2 solution
- Solution for GC-rich templates and for minimizing background
Select the optimal buffer system for the particular application. With the detergent-containing buffer B, the yields of the PCR reaction can be optimized. The BD buffer without Tween-20 is recommended for detergent-sensitive, downstream applications.
Packaging size: 100µl - 500µl
Shipping temperature: room temperature
Storagetemperatur: -20° Celsius
It's easy with the S-solution of Bio&SELL - the results of PCR templates with high GC content can be significantly improved by the addition of the S-solution. Even with unwanted by-products in the PCR reaction, the S-solution contributes to background minimization.
The separately packaged MgCl2 solution allows the variation of the Mg 2+ ion concentration in your PCR reaction, thereby providing for further optimization of the specific PCR reactions.
For educational purposes, you can impressively demonstrate to your students the Mg 2+ dependence of enzyme activity of DNA polymerase.
Application & Quality Control
Primer extension reactions: the enzyme is free of nicking and primers activities as well as of exonucleases and non-specific endonucleases. SDS / PAGE: 95 kD band, purity> k98%. Activity and stability were tested by PCR. The error rate per cycle per Nukeotid is 8.3x10-5 1.2x104 accuracy. The half-life at 95 °C is 90 min.
Storage and dilution buffer
50% glycerol (v / v), 20 mM Tris HCl (pH 8.7 at 25 °C), 100 mM KCl, 0.1 mM EDTA and stabilizers.
One unit is the amount of enzyme that is needed to convert 10 nmol of dNTP in 30 min at 74 ° C in an acid-insoluble form.