The Bio&SELL HEK (human embryonic kidney) 293 media serum-free are particularly rich media. Bio&SELL offers optimized media for the adherent and suspension culture of HEK-293 cells.
Bio&SELL HEK 293 medium serum-free supports:
- fast cell attachment
- guarantees high proliferation rates
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| Product | Article-No. | Quantity |
|---|---|---|
|
Bio&SELL HEK 293 Medium serum-free for adherent cell culture ready-to–use medium for the cultivation of HEK 293-cells |
BS 23.525.0500A | 1 x 500ml |
|
Bio&SELL HEK 293 Medium serum-free for suspension cell culture ready-to–use medium for the cultivation of HEK 293-cells |
BS 23.525.0500S | 1 x 500ml |
Application of Bio&SELL HEK 293 Medium
- Purification of recombinant proteins from the cell culture supernatant
- In the development and production of viral vaccines & chemotherapeutics
- Production of rekomcinant Adenovirus-vectors
- On request we will send you an This email address is being protected from spambots. You need JavaScript enabled to view it.
Adaption to serum-free culture
Usually, no special adaption of HEK cells is needed when changing from a medium containing serum to a serum free medium. In some cases, a gradual reduction of serum is necessary to adapt the cells to the culture under serum free conditions.
We recommend to add serum to the serum-free medium and then reduce the amount of serum step by step. The success of the adaption also depends on the cell vitality and density. Best results are obtained with cells in the logarithmic growth phase. Seeding the cells with a higher density supports the adaption.
When working with trypsine, a careful removal of the remaining trypsine or the application of a trypsine-inhibitor is essential, since there are no serum components to neutralize the trypsine activity.