Neuro-BS basal medium
Neuro-BS basal medium is a basic medium for the in vitro cultivation of neuronal cells (e.g. hippocampus, cortex and many other cells of the central nervous system). It is suitable for long-term cultivation of neuronal cell cultures and maintains pure populations of neuronal cells without the need for a “feeder layer” of astrocytes.
Our Neuro-BS basal medium uses a sodium bicarbonate buffer system and requires a CO2 atmosphere of 5 to 10 % to maintain physiological pH. Since our medium does not contain glutamate, you should add glutamate to the primary culture. We also recommend adding fetal calf serum (FBS) or (for serum-free culture) our supplements specially developed for this medium before use.
Our special supplements for serum-free cultivation of neuronal cells contain proteins such as insulin and transferrin, albumins and hormones. They enable the long-term maintenance of the normal cell phenotype and growth of neuronal cells without a feeder layer of astrocytes. You receive the supplements in 50x or 100x concentrated form.
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