Human serum AB male
Our human serum AB male is derived exclusively from the plasma of male donors with blood type AB and is free of anti-A and anti-B antibodies. For this reason, it does not react with other blood types, and unwanted immune reactions are minimized. Calcium chloride is added to the plasma to induce coagulation, and cellular and solid components of the plasma are removed by centrifugation. The AB serum is then washed, concentrated, and filtered. Our human AB serum contains numerous growth factors, vitamins, nutrients, trace elements, and transport factors. Pooled serum from donors of the same sex reduces variations in the final product. As a result, this AB serum consistently maintains high quality and is particularly recommended for immunological and hematopoietic studies.
Our human serum is derived from plasma or whole blood donations exclusively from healthy donors at certified institutions in Europe or the United States. Each individual donation is screened for viral infections, verified, and documented. This ensures maximum safety and transparency for you.
Properties
- Guaranteed to be free of anti-A and anti-B antibodies!
- Improved reproducability - Pooled serum from one donor sex reduces variation in the final product
- No animal components - Created in vivo conditions for human in vitro applications
- High safety - Every whole blood or plasma donation is intensively tested for infections
Good to know
Human whole blood consists of 55% plasma and 45% solid blood components (red blood cells, white blood cells, and platelets). To isolate plasma, anticoagulants are added to prevent the whole blood from clotting. Centrifugation is used to separate the solid and cellular components. The resulting plasma contains suspended blood cells, nutrients, hormones, minerals, and proteins such as clotting factors. Human serum corresponds to the liquid portion of plasma, without clotting factors or cellular components. It consists of nutrients, minerals, hormones, antigens, antibodies, and other proteins. To obtain serum, whole blood is either allowed to clot naturally, or isolated plasma is treated with calcium chloride. Cellular and solid components are removed by centrifugation.
To simulate in vivo conditions for in vitro cell culture, serum is often added to the cell culture medium. Due to its unique composition, fetal bovine serum (FBS) is one of the most important supplements for cell proliferation in in vitro cell culture. However, for the cultivation of human cells, human serum may be more suitable than FBS, since both the serum and the cells being cultured belong to the same species. If you wish to switch your cells from FBS to human serum, they should be gradually acclimated to the new serum. To do this, first culture the cells for 4–6 days using a 10% serum mixture consisting of 5% FBS and 5% human serum. Afterward, you can permanently switch the cell culture to human serum.
Which human sera we offer:
Human serum - Coagulated with CaCl₂
Human serum is the liquid obtained after coagulation factors have been removed from human blood plasma. Calcium chloride is added to the human plasma to induce coagulation. Cellular and solid components of the plasma are removed by centrifugation. The human serum is then washed, concentrated, and filtered.
Human AB serum (Male) - Guaranteed free of anti-A and anti-B antibodies
Our human AB serum contains only plasma from male donors with blood type AB and is free of anti-A and anti-B antibodies. For this reason, it does not react with other blood types, and unwanted immune reactions are minimized. Human AB serum is particularly recommended for immunological and hematopoietic studies.
Human off-the-clot serum - Free of anticoagulants, naturally clotted
Human off-the-clot serum is derived from human whole blood and collected without anticoagulants. Clotting occurs naturally at room temperature. The blood clot is then removed, and the serum is purified using depth and membrane filters. The serum contains many growth factors, vitamins, nutrients, trace elements, and transport factors.
Human serum AB male off-the-clot - Guaranteed free of anti-A and anti-B antibodies, naturally clotted
This human off-the-clot serum is collected exclusively from male donors with blood type AB and is free of anti-A and anti-B antibodies. The off-the-clot AB serum is derived from whole blood and collected without anticoagulants. Clotting occurs naturally at room temperature. The serum contains many growth factors, vitamins, nutrients, trace elements, and transport factors.
FAQs
What is the composition of human AB serum?
Human AB serum contains all the components of blood plasma (except for clotting factors), such as proteins, electrolytes, hormones, and nutrients. Since the plasma is sourced exclusively from AB-blood-type donors, it does not contain anti-A or anti-B antibodies.
What are the advantages of human serum compared to FBS?
The advantage of human serum over fetal bovine serum (FBS) is that it contains no animal components that could interfere with cell culture. Human cells thus encounter conditions that are nearly identical to those found in nature, even in in vitro cell culture.
What is human serum (definition)?
Human serum is derived from human blood plasma. Blood serum refers to the aqueous portion of plasma that does not clot or, after blood clotting has occurred, remains as a yellowish liquid.
Why is only male serum used?
Pooled AB serum from a single donor sex reduces variations in the final product and significantly increases the reproducibility of your experiments.
At what concentration should human AB serum be used?
We recommend adding human AB serum to your cell culture medium at a concentration between 5% and 20%.
How are the cells adapted to human AB serum?
We recommend a gradual adaptation process. Culture the cells for the first 4–6 days using a 10% mixture of FBS and human serum (= 5% human serum and 5% FBS). Afterward, you can permanently switch the cell culture to human serum.