SCRIPTUM First Strand
SCRIPTUM First Strand - cDNA Synthesis Kit enables the reverse transcription (RT) of different RNA templates with subsequent polymerase chain reaction (PCR). Our kit contains all necessary components for efficient cDNA synthesis and has a high efficiency for a wide range of primer-template combinations.
SCRIPTUM First Strand contains both random hexamer primers and oligo (dT)20 primers for highly flexible first strand cDNA synthesis. With a premium quality reverse transcriptase, ultra-pure dNTPs and an optimized reaction buffer, the kit also ensures superior results with highest reproducibility. The SCRIPTUM First Strand Reverse Transcriptase is characterized by reduced RNase H activity and enables increased synthesis efficiency with high cDNA yields in only 5 to 20 min. We recommend our SCRIPTUM First Strand Kit for cDNA synthesis of RNA templates with 100 bp to 17 kb (at an RNA input of 10 μg). In addition, our kit contains a DNase enzyme mix for the efficient digestion of genomic DNA.
The kit contains both random hexamer primers and oligo (dT)20 primers for highly flexible first strand cDNA synthesis. Optionally, gene-specific primers can also be used. For one-step RT-PCRs with gene-specific primers we recommend our 'SCRIPTUM High Precise' Kit.
Good to know
The principle of RT-PCR consists of the synthesis of cDNA by reverse transcription (RT) of an RNA template and subsequent amplification of the cDNA by polymerase chain reaction (PCR).
The first step of RT-PCR consists of converting RNA into complementary DNA (cDNA). This is done by the enzyme reverse transcriptase, which uses the RNA as a template to synthesise the cDNA. Once the cDNA has been synthesised, PCR is carried out to amplify the cDNA. Specific primers are used that bind to the cDNA. New DNA strands are synthesised with the help of a DNA polymerase.
RT-PCR is used in gene expression analysis, in the diagnosis of infectious diseases and in basic research.
Different RT-PCR types:
- Quantitative RT-PCR (qRT-PCR): This method enables the quantitative determination of the amount of RNA in a sample. It uses fluorescent dyes or probes to monitor amplification in real time.
- Semi-quantitative RT-PCR: This method allows an approximate quantification of the RNA by analysing the amplification in different cycles, but not in real time.
- Multiplex RT-PCR: Several target genes are amplified in a single reaction, which saves time and material.