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5x HS Multiplex PCR-Mastermix

For parallel amplification of multiple fragments in a single PCR.

The 5x Hot-Start Multiplex PCR-Mastermix is a ready-to-load solution for multiplex and normal Hot-Start PCRs. 

  • The Mastermix contains all the necessary reagents except for template DNA, primers and water. 
  • The process takes place in a single reaction vessel.
  • The reactions do not need to be pipetted on ice.
  • The mix is at room temperature for 1 month, at 4 ° C up to 6 months and at - 20 °C stable at least 12 months.
  • The dye contained in the mix facilitates pipetting and at the same time serves to visualize the electrophoretic separation.
  • The generated PCR products can be used for TA cloning