Leibovitz's L-15 medium was developed forcell cultivation in environments without CO2 stabilization. This cell culture medium contains phosphates and a high content of free amino acids as a buffer system in contrast to the generally used buffer substance sodium hydrogen carbonate. Leibovitz's L-15 medium is ideally suited for the proliferation of HEP-2 monkey kidney cells, human nerve cells and primary cells of embyronal or adult human tissue explants.
Philipp Straube, Anja Beckers, Ulrich W. H. Jany, Florian Bergmann, Timo H.-W. Lüdtke, Carsten Rudat, Mark-Oliver Trowe, Imke Peters, Maximilian G. Klopf, Tamrat M. Mamo and Andreas Kispert
The Company of Biologists | Development (2025) 152, dev204214