Leibovitz's L-15 medium was developed forcell cultivation in environments without CO2 stabilization. This cell culture medium contains phosphates and a high content of free amino acids as a buffer system in contrast to the generally used buffer substance sodium hydrogen carbonate. Leibovitz's L-15 medium is ideally suited for the proliferation of HEP-2 monkey kidney cells, human nerve cells and primary cells of embyronal or adult human tissue explants.
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Leibovitz's L-15
(rich in free amino acids, without glutamine, without NaHCO3)