qPCR Mastermix Probe No Rox is optimized for probe-based real-time PCRs and contains all necessary components for quantitative PCR except template DNA, primer and probe. qPCR Mastermix Probe No Rox is 5x concentrated and contains optimized components such as our Hot-Start Taq DNA Polymerase in a proprietary buffer system that allows the detection of "low-copy-number targets".