BSK-H Medium
BSK-H medium is a specific, very nutrient-rich ready-to-use cell culture medium for the cultivation or isolation of spirochaetes (such as Borrelia spec., especially B. burgdorferi and B. hermsii). Our BSK-H medium is based on the Connaught Medical Research Laboratories (CMRL) medium 1066 and is additionally enriched to meet the nutritional requirements of freshly isolated spirochetes.
The special formulation of the medium is characterized by a very high content of proteins and peptides, a richness of nucleosides (nucleobase and pentose, without phosphate residues) and a very high content of vitamins. Glucose serves as an energy source for the cells. BSK-H medium uses the buffer substance HEPES to maintain the pH value and guarantee long-term stability. Our BSK-H medium also contains N-acetyl-D-glucosamine, which is the essential element of bacterial peptidoglycan.
Through specific galenics and precise production processes, we guarantee you a BSK-H medium of the highest quality and purity from batch to batch. This enables you to achieve constant and reproducible cell growth of your spirochaetes. For guaranteed success of your in vitro cell culture, we recommend supplementing the BSK-H medium with 3-8% rabbit serum.
Article list
| Products | Article number | Quantity | Shop |
|---|---|---|---|
|
BSK-H Medium - with L-glutamine
(with 6 g/l D-glucose, with 6 g/l HEPES, with phenol red)
|
BS.2.12ML.100
|
100 ml
| |
|
BS.2.12ML.500
|
500 ml
| ||
|
BSK-H Medium - without glutamine
(with 6 g/l D-glucose, with 6 g/l HEPES, with phenol red)
|
BS2.12OL.100
|
100 ml
| |
|
BS.2.12OL.500
|
500 ml
|
Good to know
The use of BSK-H medium for the cultivation of Borrelia is recommended by the DMSZ.
Our ready-to-use BSK-H medium does not contain rabbit serum and should be supplemented with 3-8% rabbit serum.
Important: We strongly advise you not to purchase BSK-H Medium pre-supplemented with rabbit serum.As the formulation of the rabbit serum contained is unknown, you have no means of intervening or manipulating your Borrelia culture in the event of growth difficulties. It is also not possible to reproduce your experiments with different batches. It is better to add rabbit serum from a known batch yourself to ensure a consistent formulation. (e.g. RAB.SE.0100)