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VLE DMEM

Very Low Endotoxin (VLE) DMEM is a standardized cell culture medium with a greatly reduced endotoxin content. The low endotoxin content reduces batch variations of the medium and increases the reproducibility of the experiments. DMEM is a modified Eagle medium and is characterized by its fourfold content of vitamins and amino acids. Its high nutrient content makes it very suitable for cultivating a wide range of adherent cell types such as mammalian cells, insect cells and plant cells.

Article list

ProductsArticle numberQuantityShop
VLE DMEM
Very Low Endotoxin, with stable glutamine, with 3.7 g/l NaHCO3
BS.FG1445
500 ml

Good to know

Endotoxins belong structurally to the lipopolysaccharides (LPS) and are components of the outer cell membrane of gram-negative bacteria or cyanobacteria. They are released by living bacteria through the cleavage of vesicles or upon death through the disintegration of the outer cell wall and trigger strong cellular and immunological reactions. Bacteria are inactivated by various sterilization processes, but endotoxins are very heat-stable and usually even survive sterilization. In cell culture, endotoxins lead to undesired immunological cellular reactions, a negative influence on transfection and they reduce both the transfection efficiency and the reproducibility of the experiments.

Publications

Differences in Starvation-Induced Autophagy Response and miRNA Expression Between Rat Mammary Epithelial and Cancer Cells: Uncovering the Role of miR-218-5p

Mateusz Gotowiec, Antoni Smolinski, Katarzyna Marcinkowska, Wiktor Pascal, Paweł Krzysztof Włodarski
Cancers 17 (2025), 2446

Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation

Kami Pekayvaz, Christoph Gold, Parandis Hoseinpour, Anouk Engel, Alejandro Martinez-Navarro, Luke Eivers, Raffaele Coletti, Markus Joppich, Flavio Dionisio, Rainer Kaiser, Lukas Tomas, Aleksandar Janjic, Maximilian Knott, Fitsumbirhan Mehari, Vivien Polewka, Megan Kirschner, Annegret Boda, Leo Nicolai, Heiko Schulz, Anna Titova, Badr Kilani, Michael Lorenz, Geunter Fingerle-Rowson, Richard Bucala, Wolfgang Enard, Ralf Zimmer, Christian Weber, Peter Libby, Christian Schulz, Steffen Massberg, Konstantin Stark
Immunity 56 (2023), 2325–2341