BioColl® (for murine Lymphocytes)

Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to produce aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.086 g/ml is ideal for the isolation of mouse lymphocytes.

For optimal pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

Density: 1.086 g/ml
For the isolation of murine lymphocytes
Aqueous solution of highly cross-linked polysucrose

Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or in the production of antibodies.

BioColl® is a registered trademark of Bio&SELL GmbH.