Reverse Transcriptases for cDNA synthesis
Reverse transcriptases are enzymes that transcribe RNA into complementary DNA (cDNA). This step forms the basis for many molecular biology applications - such as RT-PCR, RT-qPCR, or the subsequent analysis of RNA transcripts. Which reverse transcriptase is suitable for your experiment depends on your experimental setup, the RNA sample, and subsequent applications.
At Bio&SELL, you’ll find the SCRIPTUM-RT series, which includes three variants of reverse transcriptases. Choose the right enzyme for your experimental setup:
Our SCRIPTUM reverse transcriptases (RT):
- SCRIPTUM-37 RT - M-MuLV reverse transcriptase with an optimal temperature of 37°C
- SCRIPTUM-55 RT - M-MuLV reverse transcriptase with thermal stability up to 55°C
- SCRIPTUM-60 RT - M-MuLV reverse transcriptase with high thermal stability up to 60°C
FAQs
What does a reverse transcriptase do?
A reverse transcriptase synthesizes complementary DNA (cDNA) from an RNA template. This cDNA can then be analyzed, for example, in RT-PCR or RT-qPCR applications.
What is cDNA used for?
cDNA serves as a more stable DNA copy of an RNA template and can be used as starting material for subsequent molecular biology analyses.
Which reverse transcriptase should I choose?
Select the appropriate reverse transcriptase for your specific experiment. Be sure to consider the enzyme’s thermostability, RNA affinity, and purity.
