Low Melt Agarose (or low melting point agarose) is an agarose with a comparatively low melting point. It is ideal for fragment separation between 80 bp and 25,000 bp.
The melting point is guaranteed to be < 66°C, lower than that of universal agarose.
Excellently suited for preparative gel electrophoresis.
The excised gel piece with the desired DNA fragment can be melted at a lower temperature, thus allowing the DNA to be obtained more gently.
Like all our agaroses, each batch of Bio&SELL low melting point agarose undergoes strict quality controls with regard to all relevant parameters.
Guaranteed properties of Universal Agarose:
Gel strength (1.0%):> 200 g/cm2
Gelling temperature (1.5 %): < 31 °C
Melting temperature (1.5%): <66 °C
Electroendosmosis (-mr): 0.05-0.14
Sulfate content: < 0.10 %
Water content: < 10.0 %
DNA binding: not detectable
DNase and RNase activity: not detectable
Preparation of a standard agarose gel:
For 100 ml of a 1% agarose gel, 1 g of agarose is prepared in 100 ml of electrophoresis buffer. After dissolving the agarose by heating and a cooling phase, you can cast the gel.