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qPCR Mastermix EvaGreen® No Rox

qPCR Mastermix EvaGreen® No Rox is optimized for EvaGreen®-based real-time qPCRs and contains all necessary components for quantitative PCR except template DNA and primers. The 5x concentrated kit is highly specific and provides reproducible results for low copy DNA targets, long template or GC-rich DNA targets.

This kit does not contain the reference dye Rox.

Article list

ProdukteArtikelnummerMengeShop
qPCR Mastermix EvaGreen® No Rox
5x concentrated
BS76.590.0200
50 reactions
BS76.590.1000
250 reactions
BS76.590.5000
1250 reactions

Good to know

EvaGreen® is a DNA-binding dye with many properties that show many advantages over its use with SYBR® Green I for quantitative PCR. In addition to a similar spectrum, there are three important advantages that make EvaGreen® stand out compared to SYBR® Green I: EvaGreen® exhibits lower PCR inhibition, is an extremely stable dye and is neither mutagenic nor cytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers - simply select the default setting for SYBR® Green or FAM!

Devices and device settings

Compatible with all common plate real-time cyclers, regardless of Rox requirements.
For all “Standard Cyling Mode”, “Standard” and “Fast” Real-Time Cyclers.
EvaGreen® is compatible with all common real-time PCR cyclers - simply select the default setting for SYBR® Green or FAM!

Application

  • Detection and quantification of DNA and cDNA targets
  • Profiling of gene expression
  • Microbial Detection
  • Determination of viral loads

*EvaGreen® is a registered trademark and licensed for sale by BIOTIUM, Inc., Hayward, CA, USA.

This product is provided under an agreement with BIOTIUM, INC., and the manufacture, use, sale or import of this product is subject to one or more of pending patent applications owned by BIOTIUM and ALLELOGIC. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact BIOTIUM, INC., Business Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel: 510-265-1027, Fax: 510-265-1352.

SYBR® is a regeistered rademark of Molecular Probes, Inc.

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