SCRIPTUM-60 RT

Our SCRIPTUM-60 Reverse Transcriptase (RT) (200 U/µl) is an M-MuLV reverse transcriptase that generates cDNA quickly and specifically even at high temperatures. Due to a genetic modification, the enzyme has an outstanding thermostability up to 60°C and is ideal for the reverse transcription of RNAs with extremely complex secondary structures (up to 10 kb). Our SCRIPTUM-60 RT also has a very high RNA affinity, making it ideal for use in assays with low-concentration target RNA or low-copy genes. Due to its high purity, SCRIPTUM-60 RT is also ideal for the detection of pathogens.

For best results, we offer our SCRIPTUM-60 RT with a specifically optimized buffer.

Outstanding thermostability - thermostability up to 60°C: Enables reverse transcription of complex RNA secondary structures
Very high RNA affinity - Excellent for low concentration target RNA or low copy genes
Excellent purity - Ideal for pathogen detection

Single-stranded nucleic acids often form intramolecular double strands through hydrogen bonds between complementary sections. This results in secondary structures with so-called “stems” (paired double-stranded sections) and “loops” (double-stranded sections that include single-stranded sections). RNA secondary structures can take various forms such as hairpin loops, interior loops or multi-loops.

To analyze an unstable RNA target structure, it must first be transcribed into a stable cDNA by a reverse transcriptase. However, complex RNA secondary structures make it impossible for the reverse transcriptase to attach to the RNA or to read the information. In order to resolve complex RNA secondary structures, the assay temperature must be increased. However, human enzymes begin to denature at 42°C. Our SCRIPTUM-60 RT has been genetically modified and has outstanding thermostability up to 60°C, which means that even reverse transcription of extremely complex RNA secondary structures is no longer a problem.