Human serum

• Coagulated with CaCl2 - With anticoagulants
• No animal components - Created in vivo conditions for human in vitro applications
• High safety - Every whole blood or plasma donation is intensively tested for infections
  
  

Human whole blood consists of 55% plasma and 45% solid blood components (red blood cells, white blood cells and platelets). In order to isolate plasma, anticoagulants are added to prevent the whole blood from clotting. Solid and cellular components are separated by centrifugation. The plasma then contains suspended blood cells, nutrients, hormones, minerals and proteins such as coagulation factors. Human serum corresponds to plasma fluid, without coagulation factors and cellular components. It consists of nutrients, minerals, hormones, antigens, antibodies and other proteins. To obtain serum, whole blood is either naturally clotted or isolated plasma is mixed with calcium chloride. Cellular and solid components are removed by centrifugation.

To create in vivo conditions for in vitro cell culture, serum is often added to the cell culture medium. Due to its unique composition, fetal bovine serum (FBS) is one of the most important supplements for cell proliferation in in vitro cell culture. However, human serum may be more suitable than FBS for culturing human cells, as both the serum and the cells to be cultured belong to the same species. If you want to convert your cells from FBS to human serum, they should be adapted to the new serum step by step. To do this, first cultivate the cells for 4-6 days with a 10% serum mixture consisting of 5% FBS and 5% human serum. You can then finally switch the cell cultivation to human serum.

Which human sera we offer:

Human Serum - Coagulated with CaCl₂
Human serum is the liquid obtained after coagulation factors have been removed from human blood plasma. Calcium chloride is added to the human plasma to induce coagulation. Cellular and solid components of the plasma are removed by centrifugation. The serum is then washed, concentrated, and filtered.

Human Serum AB Male - Guaranteed Free of Anti-A and Anti-B Antibodies
Human Serum AB Male contains only plasma from male donors with blood type AB and is free of anti-A and anti-B antibodies. For this reason, it does not react with other blood types, minimizing the risk of adverse immune reactions. This serum is particularly recommended for immunological and hematopoietic studies.

Human Serum Off-the-clot – without anticoagulants, naturally clotted
Human Serum Off-the-clot AB Male is derived from human whole blood and collected without anticoagulants. Clotting occurs naturally at room temperature. The blood clot is then removed, and the serum is purified using depth and membrane filters. The serum contains many growth factors, vitamins, nutrients, trace elements, and transport factors.

Human Serum Off-the-clot AB Male - guaranteed to be free of anti-A and anti-B antibodies, naturally clotted
Human Serum Off-the-clot is collected exclusively from male donors with blood type AB and is free of anti-A and anti-B antibodies. The serum is derived from whole blood and collected without anticoagulants. Clotting occurs naturally at room temperature. The serum contains many growth factors, vitamins, nutrients, trace elements, and transport factors.

FAQs

What is the composition of human serum?
Human serum contains all the components of blood plasma (except for clotting factors), such as proteins, electrolytes, hormones, and nutrients.

What are the advantages of human serum over FBS?

• No interfering animal components
• Human cells encounter conditions as close to natural as possible, even in in vitro cell culture

What is human serum (definition)?
Human serum is derived from human blood plasma. Blood serum refers to the aqueous portion of plasma that does not clot or, after blood clotting has occurred, remains as a yellowish liquid.

In which fields is human serum used?
Cell therapy, immunotherapy research, tissue engineering, etc.

At what concentration should human serum be used?
For our human sera, we recommend a concentration between 5% and 20% in cell culture.

How do the cells adapt to human serum?
We recommend a gradual adaptation. Cultivate the cells for the first 4-6 days using a 10% mixture of FBS and human serum (5% human serum and 5% FBS). Afterward, you can cultivate the cells with 5-20% human serum.