FBS Low Endotoxin

Our FBS low endotoxin is characterized by a low endotoxin content (up to < 1 EU/ml). It undergoes a rigorous selection process and, following additional treatment, contains virtually no endotoxins of bacterial origin. Low endotoxin fetal bovine serum (FBS) is suitable for the cultivation of sensitive cell lines, primary cell cultures, hybridoma cloning, and transfections. Endotoxins very often have negative effects on the success of a transfection. Our low endotoxin FBS is characterized by increased transfection efficiencies and improved reproducibility of experiments due to reduced batch-to-batch variations.

We offer three different low endotoxin products: Ultra Low Endotoxin FBS, Very Low Endotoxin FBS, and Low Endotoxin FBS

Structurally, endotoxins belong to the class of lipopolysaccharides (LPS) and are components of the outer cell membrane of Gram-negative bacteria or cyanobacteria. They are released by living bacteria through the detachment of vesicles or, upon cell death, through the breakdown of the outer cell wall, and trigger strong cellular and immunological reactions. Bacteria are inactivated by various sterilization methods, but endotoxins are highly heat-stable and usually survive even sterilization. In cell culture, endotoxins have a negative impact on transfection and reduce both transfection efficiency and the reproducibility of experiments.