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DMEM/F-12

DMEM/F-12 is a 1:1 mixture of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12). This formulation is used as a basal medium for the cultivation of a wide range of mammalian cells (e.g. MDCK cells, glial cells, fibroblasts, human endothelial cells, rat fibroblasts). DMEM is a modified Eagle medium and is characterized by its quadruple content of vitamins and amino acids. Ham's F-12 is based on Ham's F-10 medium and is a basal medium developed for serum-free cell culture.

Since the formulation of DMEM/F-12 does not contain any proteins or growth factors, 10% fetal calf serum (FBS) should be added to the medium for cell culture.

Article list - Liquid media

ProductsArticle numberQuantityShop
DMEM/F-12 - without glutamine
(with 3.2 g/l D-glucose, with 1.2 g/l NaHCO3, with phenol red)
BS.F4815
500 ml
DMEM/F-12 - with stable glutamine
(with 3.2 g/l D-glucose, with 1.2 g/l NaHCO3, with phenol red)
BS.FG4815
500 ml

Article list - Powder media

ProductsArticle numberQuantityShop
DMEM/F-12 - with L-glutamine
Powder medium
BS.T481-01
for 1 liter
DMEM/Ham's F-12 - with L-glutamine
Powder medium
BS.T481-10
for 10 liter

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