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High Resolution Agarose

Our high-resolution agarose is specially optimized for the separation of nucleic acids in the size range 20 - 800 bp. Due to the medium melting temperature, an extremely high resolution is achieved in the size range mentioned above.

Typical applications are investigations of:

  • RFLP ((Restriction fragment length polymorphism)
  • AMPFLP (amplified fragment length polymorphism)
  • HVR (Hyper-Variable Regions)
  • VNTR (variable numbers of tandem repeats)
  • STR (short tandem repeats)
  • Tri- and tetra-nucleotide repeats
  • Exact analysis of PCR products
  • DNA identification

This makes Bio&SELL high-resolution agarose the convenient alternative to polyacrylamide gels.

Advantages

  • Significantly cheaper than polyacrylamide gels
  • No handling of toxic substances such as PAA

Article list

ProductsArticle numberQuantityShop
High Resolution Agarose
BS20.48.025
25 g
BS20.48.100
100 g
BS20.48.250
250 g

Guaranteed properties of the high-resolution agarose:

  • Gel strength (1.0%): > 300 g/cm2
  • Gelling temperature (1.5 %): < 35 °C
  • Electroendosmosis (-mr): < 0.5
  • Water content: < 10.0 %
  • DNA binding: not detectable
  • DNase and RNase activity: not detectable

The specific values of each batch are certified in the enclosed data sheet.

Fragment size
(bp) Final concentration of agarose (%)
x g agarose in 100ml
1x TAE buffer Final concentration of agarose (%)
x g agarose in 100ml
1x TBE buffer
150 – 800 2.0 1.8
100 – 600 3.0 2.0
50 – 250 4.0 3.0
20 – 130 5.0 4.0
< 80 - 5.0

Preparation of a standard agarose gel:

For 100 ml of a 1% agarose gel, 1 g of agarose is prepared in 100 ml of electrophoresis buffer. After dissolving the agarose by heating and cooling, you can cast the gel.

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