Universal Medium

Our universal medium is a highly versatile, serum-free medium for the cultivation of a wide range of animal and human adherent and non-adherent cell types. The formulation is based on Iscove's Modified Essential Medium (IMEM), which has been enriched with trace elements, albumins, cholesterol, soy lipids and vitamins. The ready-to-use medium has no growth or attachment factors, which is why you have the opportunity (e.g. when researching specific growth factors) to investigate the effect on your cells by adding specific growth factors.

Our Universal Medium has been successfully tested on the following cells:
Hybridomas, lymphocytes, macrophages, fibroblasts, melanocytes, carcinoma cells, HEK cells, HeLa cells, CHO cells, magakaryocytic cell lines (HEL, Dami, CMK), HL60 cells

In order to create in vivo conditions for cell growth in the cell culture, serum is always added to the medium. Serum serves the cells as a source of growth factors, lipids, hormones and minerals. High-quality sera are usually expensive and the composition is not precisely defined. For this reason, serum-free media are produced. These media contain additives that enable cell cultivation without the addition of serum.

Serum-free media are generally divided into

1. Non-defined serum-free media (can have undefined plant and animal components)
2. Chemically-defined serum-free media (only with components of known structure and weight)
3. Protein-free media (absolutely free of proteins; usually with hydrolysates)
4. Xeno-free media (free of proteins of non-human origin)

The use of serum-free media offers more consistency and reproducibility in cell culture. This makes cell cultivation more controllable and minimizes the risk of contamination. It also reduces costs by eliminating serum and simplifies downstream protein purification (protein yield is often higher).