BioColl® with HEPES (for lymphocytes)

Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.077 g/ml is ideal for the isolation of lymphocytes. Our product also contains 10 mM HEPES.

For optimal pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

Density: 1.077 g/ml
With 10 mM HEPES
For the isolation of human lymphocytes
Centrifugation medium made from highly cross-linked polysucrose

Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or the production of antibodies.

Platelet density 1.040 - 1.060 g/ml
Monocyte density 1.059 - 1.068 g/ml
Lymphocyte density 1.066 - 1.077 g/ml
Basophil density 1.075 - 1.081 g/ml
Neutrophil density 1.080 - 1.099 g/ml
Eosinophil density 1.088 - 1.096 g/ml
Erythrocyte density 1.090 - 1.110 g/ml

BioColl® is a registered trademark of Bio&SELL GmbH.