BioColl® with HEPES (for lymphocytes)
Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.077 g/ml is ideal for the isolation of human lymphocytes. Our product also contains 10 mM HEPES.
For optimal pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.
Properties
Density: 1.077 g/ml
With 10 mM HEPES
For the isolation of human lymphocytes
Centrifugation medium made from highly cross-linked polysucrose
Good to know
Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or the production of antibodies.
What are the densities of my cells/cell particles?
| Thrombocytes | Density 1.040 - 1.060 g/ml |
| Monocytes | Density 1.059 - 1.068 g/ml |
| Lymphocytes | Density 1.066 - 1.077 g/ml |
| Basophiles | Density 1.075 - 1.081 g/ml |
| Neutrophiles | Density 1.080 - 1.099 g/ml |
| Eosinophiles | Density 1.088 - 1.096 g/ml |
| Erythroctyes | Density 1.090 - 1.110 g/ml |
Which BioColl® separation solution do I need for my cells?
| Cell type | BioColl® Separation Solution | Article Number |
| Human lymphocytes | Density 1.077 g/ml | BS.L6115 (or BS.L.6715) |
| Human monocytes | Density 1.068 g/ml + 1.077 g/ml | BS.L6815 + BS.L6115 |
| Human granulocytes | Density 1.077 + 1.1 g/ml | BS.L6115 + BS.L6155 |
| Murine lymphocytes | Density 1.086 g/ml | BS.L6121 |