BioColl® (for Lymphocytes)

Our BioColl® separation solution, with a density of 1.077 g/mL, is ideal for isolating human lymphocytes using density gradient centrifugation. The aqueous BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer creates a density gradient that separates cells and cell components in the centrifugal field based on their size, shape, and density. The appropriate density of the BioColl® separation solution is selected based on the particle type. A density of 1.077 g/mL is ideal for PBMC isolation, particularly for the isolation of human lymphocytes.

To achieve optimal pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

Density: 1.077 g/ml
For the isolation of human lymphocytes
Centrifugation medium made from highly cross-linked polysucrose

Cells (e.g., PBMCs) are isolated using a method known as density gradient centrifugation. This physical process is based on separating macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic testing, gene expression studies, or antibody production.

*BioColl® is a registered trademark of Bio&SELL.