BioColl® (for Lymphocytes)

Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.077 g/ml is ideal for the isolation of human lymphocytes.

For optimum pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

Product information
Application

Properties

Density: 1.077 g/ml
For the isolation of human lymphocytes
Centrifugation medium made from highly cross-linked polysucrose

Article list

Products	Article number	Quantity
BioColl® (lymphocytes) Separating solution - density 1.077 g/ml	BS.L6113	100 ml
	BS.L6115	500 ml
	BS.L.6117	1000 ml

Good to know

Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or the production of antibodies.

What are the densities of my cells/cell particles?

Thrombocytes	Density 1.040 - 1.060 g/ml
Monocytes	Density 1.059 - 1.068 g/ml
Lymphocytes	Density 1.066 - 1.077 g/ml
Basophiles	Density 1.075 - 1.081 g/ml
Neutrophiles	Density 1.080 - 1.099 g/ml
Eosinophiles	Density 1.088 - 1.096 g/ml
Erythroctyes	Density 1.090 - 1.110 g/ml

Which BioColl® separation solution do I need for my cells?

Cell type	BioColl® Separation Solution	Article Number
Human lymphocytes	Density 1.077 g/ml	BS.L6115
Human monocytes	Density 1.068 g/ml + 1.077 g/ml	BS.L6815 + BS.L6115
Human granulocytes	Density 1.077 + 1.1 g/ml	BS.L6115 + BS.L6155
Murine lymphocytes	Density 1.086 g/ml	BS.L6121

*BioColl® is a registered trademark of Bio&SELL.

Product information

Category:	BioColl® Separation Solutions
Density:	1.077 g/ml
Sterilization:	Sterile filtered

Application

Isolation of human lymphocytes
PBMC isolation

Publications

Short-chain fatty acids induced lung tumor cell death and increased peripheral blood CD4+ T cells in NSCLC and control patients ex vivo
Carolin D. Thome, Patrick Tausche, Katja Hohenberger, Zuqin Yang, Susanne Krammer, Denis I. Trufa, Horia Sirbu, Joachim Schmidt, Susetta Finotto
Front. Immunol. (2024) 15: 1328263

Impact of bronchoalveolar lavage from influenza A virus diseased pigs on neutrophil functions and growth of co-infecting pathogenic bacteria
Simon Lassnig, Isabel Hennig-Pauka, Marta C. Bonilla, Matthias Mörgelin, Rabea Imker, Maren von Köckritz-Blickwede, Nicole de Buhr
Front. Immunol. (2024) 15: 1325269

Crosstalk between keratinocytes and neutrophils shapes skin immunity against S. aureus infection
Jule Focken, Birgit Schittek
Front. Immunol. (2024) 15: 1275153

CAR NK-92 cell–mediated depletion of residual TCR+ cells for ultrapure allogeneic TCR-deleted CAR T-cell products
Jonas Kath, Weijie Du, Stefania Martini, Magdi Elsallab, Clemens Franke, Laura Hartmann, Vanessa Drosdek, Viktor Glaser, Maik Stein, Michael Schmueck-Henneresse, Petra Reinke, Hans-Dieter Volk, Mohamed Abou-el-Enein, Dimitrios L. Wagner
Blood Adv (2023) 7 (15): 4124–4134

Primary ChAdOx1 vaccination does not reactivate pre-existing, cross-reactive immunity
Larissa Henze, Julian Braun, Lil Meyer-Arndt, Karsten Jürchott, Maike Schlotz, Janine Michel, Marica Grossegesse, Maike Mangold, Manuela Dingeldey, Beate Kruse, Pavlo Holenya, Norbert Mages, Ulf Reimer, Maren Eckey, Karsten Schnatbaum, Holger Wenschuh,Bernd Timmermann, Florian Klein, Andreas Nitsche, Claudia Giesecke-Thiel, Lucie Loyal, Andreas Thiel
Front. Immunol. (2023) 14: 1056525

Anti-Cancer Effects of Artesunate in Human 3D Tumor Models of Different Complexity
Marlene Niederreiter, Julia Klein, Kerstin Arndt, Jens Werner, Barbara Mayer
Int. J. Mol. Sci. (2023) 24, 7844

Precision Immunotherapy Utilizing Adapter CAR-T Cells (AdCAR-T) in Metastatic Breast Cancer Leads to Target Specific Lysis
Cansu E. Önder, Moustafa Moustafa-Oglou, Sarah M. Schröder, Andreas D. Hartkopf, André Koch, Christian M. Seitz
Cancers (2024) 16, 168

Mesaconate is synthesized from itaconate and exerts immunomodulatory effects in macrophages
Wei He, Antonia Henne, Mario Lauterbach, Eike Geißmar, Fabian Nikolka, Celia Kho, Alexander Heinz, Catherine Dostert, Melanie Grusdat, Thekla Cordes, Janika Härm, Oliver Goldmann, Anouk Ewen, Charlène Verschueren, Julia Blay-Cadanet, Robert Geffers, Hendrikus Garritsen, Manfred Kneiling, Christian K. Holm, Christian M. Metallo, Eva Medina, Zeinab Abdullah, Eicke Latz, Dirk Brenner, Karsten Hiller
Nat Metab. (2022) 4(5): 524–533

The Polarity and Specificity of Antiviral T Lymphocyte Responses Determine Susceptibility to SARS-CoV-2 Infection in Patients with Cancer and Healthy Individuals
Jean-Eudes Fahrner, Imran Lahmar, Anne-Gaëlle Goubet, Yacine Haddad, Agathe Carrier, Marine Mazzenga, Damien Drubay, Carolina Alves Costa Silva, Lyon COVID Study Group, Eric de Sousa, Cassandra Thelemaque, Cléa Melenotte, Agathe Dubuisson, Arthur Geraud, Gladys Ferrere, Roxanne Birebent, Camille Bigenwald, Marion Picard, Luigi Cerbone, Joana R. Lérias, Ariane Laparra, Alice Bernard-Tessier, Benoît Kloeckner, Marianne Gazzano, François-Xavier Danlos, Safae Terrisse, Eugenie Pizzato, Caroline Flament, Pierre Ly, Eric Tartour, Nadine Benhamouda, Lydia Meziani, Abdelhakim Ahmed-Belkacem, Makoto Miyara, Guy Gorochov, Fabrice Barlesi, Alexandre Trubert, Benjamin Ungar, Yeriel Estrada, Caroline Pradon, Emmanuelle Gallois, Fanny Pommeret, Emeline Colomba, Pernelle Lavaud, Marc Deloger, Nathalie Droin, Eric Deutsch, Bertrand Gachot, Jean-Philippe Spano, Mansouria Merad, Florian Scotté, Aurélien Marabelle, Frank Griscelli, Jean-Yves Blay, Jean-Charles Soria, Miriam Merad, Fabrice André, Juliette Villemonteix, Mathieu F. Chevalier, Sophie Caillat-Zucman, Florence Fenollar, Emma Guttman-Yassky, Odile Launay, Guido Kroemer, Bernard La Scola, Markus Maeurer, Lisa Derosa, Laurence Zitvogel
Cancer Discov (2022) 12:958–83

GLP-2 Is Locally Produced From Human Islets and Balances Inflammation Through an Inter-Islet-Immune Cell Crosstalk
Wei He, Osmond D. Rebello, Antonia Henne, Fabian Nikolka, Thomas Klein, Kathrin Maedler
Front. Endocrinol. (2021) 12: 697120