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BioColl® (for Lymphocytes)

Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.077 g/ml is ideal for the isolation of human lymphocytes.

For optimum pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

Properties

Density: 1.077 g/ml
For the isolation of human lymphocytes
Centrifugation medium made from highly cross-linked polysucrose

Article list

ProductsArticle numberQuantityShopDS
BioColl® (lymphocytes)
Separating solution - density 1.077 g/ml
BS.L6113
100 ml
BS.L6115
500 ml
BS.L.6117
1000 ml

Good to know

Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or the production of antibodies.

What are the densities of my cells/cell particles?

Thrombocytes          Density 1.040 - 1.060 g/ml
Monocytes Density 1.059 - 1.068 g/ml
Lymphocytes Density 1.066 - 1.077 g/ml
Basophiles Density 1.075 - 1.081 g/ml
Neutrophiles Density 1.080 - 1.099 g/ml
Eosinophiles Density 1.088 - 1.096 g/ml
Erythroctyes Density 1.090 - 1.110 g/ml

  

Which BioColl® separation solution do I need for my cells?

 Cell type BioColl® Separation Solution Article Number
Human lymphocytes          Density 1.077 g/ml BS.L6115
Human monocytes Density 1.068 g/ml + 1.077 g/ml BS.L6815 + BS.L6115
Human granulocytes Density 1.077 + 1.1 g/ml BS.L6115 + BS.L6155
Murine lymphocytes Density 1.086 g/ml BS.L6121

*BioColl® is a registered trademark of Bio&SELL.

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