Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used to isolate cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.077 g/ml is ideal for the isolation of human lymphocytes.
For optimum pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.
Properties
Density: 1.077 g/ml For the isolation of human lymphocytes Centrifugation medium made from highly cross-linked polysucrose
Article list
Products
Article number
Quantity
Shop
DS
BioColl® (lymphocytes)
Separating solution - density 1.077 g/ml
BS.L6113
100 ml
BS.L6115
500 ml
BS.L.6117
1000 ml
Good to know
Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or the production of antibodies.
What are the densities of my cells/cell particles?
Thrombocytes
Density 1.040 - 1.060 g/ml
Monocytes
Density 1.059 - 1.068 g/ml
Lymphocytes
Density 1.066 - 1.077 g/ml
Basophiles
Density 1.075 - 1.081 g/ml
Neutrophiles
Density 1.080 - 1.099 g/ml
Eosinophiles
Density 1.088 - 1.096 g/ml
Erythroctyes
Density 1.090 - 1.110 g/ml
Which BioColl® separation solution do I need for my cells?