RT-PCR Kits for RNA-Based Analyses
In reverse transcription-polymerase chain reaction (RT-PCR), RNA is enzymatically transcribed into complementary DNA (cDNA) and then amplified. The enzyme that transcribes RNA into cDNA is called reverse transcriptase. The cDNA is then amplified using a polymerase. This method is suitable for detecting RNA in a sample, e.g., in the detection of RNA viruses, in gene expression analysis, in tumor diagnostics, and in analyses combined with real-time PCRs (qPCRs).
Bio&SELL offers various RT-PCR kits:
FAQs
What is the difference between RT-PCR and RT-qPCR?
In RT-PCR, the RNA is transcribed into cDNA, and the cDNA is then amplified. Detection is generally performed using subsequent gel electrophoresis. In RT-qPCR, on the other hand, amplification is monitored in real time using dyes, enabling quantitative analysis.
What does “One Step” mean in the context of RT-PCR kits?
With a one-step kit, reverse transcription and PCR take place in a single reaction tube.
How do I choose the right RT-PCR kit?
It depends on whether you want to detect specific RNA templates or perform nonspecific first-strand synthesis. You should also consider whether you plan to perform a qualitative (RT-PCR) or quantitative (RT-qPCR) analysis, and whether you want to use a one-step or two-step approach.
