Plasmid Mini Kit

The Plasmid Mini Kit is optimized for fast and reliable isolation of plasmid DNA (“high copy” plasmid DNA, “low copy” plasmid DNA, cosmid DNA) from 0.5 - 15 ml bacterial cells in only 15 min. For this purpose, cultured bacterial cells are alkaline digested and bacterial DNA is bound and purified with the aid of silica columns. The column-based DNA purification in combination with specially formulated buffers in our kit, which are virtually harmless to health, guarantee extremely easy handling and a high level of safety. With our Plasmid Mini Kit you are able to isolate high purity bacterial DNA (A₂₆₀ /A₂₈₀: 1.7 - 2.0), which is ideal for downstream applications such as sequencing, transfection, cloning, in vitro transcription or PCR.

Fast isolation - in only approx. 15 min
Easy handling - Column-based plasmid DNA isolation
High yield - Up to 60 µg plasmid DNA
High purity - A₂₆₀/A₂₈₀ ratio: 1.7 - 2.0
High safety - No use of toxic substances such as phenol, DTT, beta-mercaptoethanol