Multiplex qPCR Mastermix No Rox is optimized for probe-based real-time PCRs of multiple DNA templates and contains all components necessary for quantitative PCR except template DNA, primer and probe. Multiplex qPCR Mastermix No Rox is 5x concentrated and contains optimized components such as our Hot-Start Taq DNA Polymerase and a specific buffer system that allows the detection of 5-10 targets.