ISF-1 Hybridoma Medium is a serum-free, ready-to-use medium specially optimized for the cultivation of hybridomas and the production of monoclonal antibodies. Our ISF-1 Hybridoma Medium is virtually free of animal or human components (< 0.1 % w/v) and contains no protein hydrolysates. The absence of animal or human components facilitates the isolation and purification of monoclonal antibodies, enables high yields and final concentrations and is suitable for the highest purity requirements. Our ISF-1 Hybridoma Medium is supplemented with human recombinant insulin and enables stable hybridoma growth and good batch-to-batch reproducibility.
(With rec. hum. insulin, with stable glutamine, with 2.4 g/l NaHCO3)
BS.F9061-01
1000 ml
Good to know
In order to create in vivo conditions for cell growth in the cell culture, serum is always added to the medium. Serum serves the cells as a source of growth factors, lipids, hormones and minerals. High-quality sera are usually expensive and the composition is not precisely defined. For this reason, serum-free media are produced. These media contain additives that enable cell cultivation without the addition of serum.
Serum-free media are generally divided into
Non-defined serum-free media (can have undefined plant and animal components)
Chemically-defined serum-free media (only with components of known structure and weight)
Protein-free media (absolutely free of proteins; usually with hydrolysates)
Xeno-free media (free of proteins of non-human origin)
The use of serum-free media offers more consistency and reproducibility in cell culture. This makes cell cultivation more controllable and minimizes the risk of contamination. It also reduces costs by eliminating serum and simplifies downstream protein purification (protein yield is often higher).